ArticleRNA (New York, N.Y.)2018
Nano LC-MS using capillary columns enables accurate quantification of modified ribonucleosides at low femtomol levels.
Article in RNA (New York, N.Y.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Harmonized nucleoside mass spectrometry enables reproducible cross-platform RNA modification quantification.bioRxiv : the preprint server for biology · 2026Article
- Translational repression of viral RNAs supports persistent arbovirus infection in mosquitoes.PLoS biology · 2026Article
- Epitranscriptomic modifications in embryonic development: insights into natural and ART-induced mechanisms and implications.Human reproduction update · 2026Review
- Coronaviruses reprogram the tRNA epitranscriptome to favor viral protein expression.Nature communications · 2026Article
- Studies on the Oxidative Damage of the Wobble 5-Methylcarboxymethyl-2-Thiouridine in the tRNA of Eukaryotic Cells with Disturbed Homeostasis of the Antioxidant System.International journal of molecular sciences · 2024Article
- Establishment of genomic RNA reference materials for BCR-ABL1 P210 measurement.Analytical and bioanalytical chemistry · 2024Article
- A highly conserved tRNA modification contributes toMicrobiology spectrum · 2024Article
- Qualitative and Quantitative Analytical Techniques of Nucleic Acid Modification Based on Mass Spectrometry for Biomarker Discovery.International journal of molecular sciences · 2024Review
- NNature communications · 2024Article
- Quantitative analysis of tRNA abundance and modifications by nanopore RNA sequencing.Nature biotechnology · 2024Article
- SWAMNA: a comprehensive platform for analysis of nucleic acid modifications.Chemical communications (Cambridge, England) · 2023Article
- Characterizing RNA modifications in the central nervous system and single cells by RNA sequencing and liquid chromatography-tandem mass spectrometry techniques.Analytical and bioanalytical chemistry · 2023Review
- Methylated guanosine and uridine modifications inRSC chemical biology · 2023Article
- Bacteriophage Infection of the Marine BacteriumMicroorganisms · 2023Article
- Permethylation of Ribonucleosides Provides Enhanced Mass Spectrometry Quantification of Post-Transcriptional RNA Modifications.Analytical chemistry · 2022Article
- Data-Independent Acquisition for the Detection of Mononucleoside RNA Modifications by Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2022Article
- Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry.Journal of visualized experiments : JoVE · 2022Article
- Single-Neuron RNA Modification Analysis by Mass Spectrometry: Characterizing RNA Modification Patterns and Dynamics with Single-Cell Resolution.Analytical chemistry · 2021Article
- Subcellular relocalization and nuclear redistribution of the RNA methyltransferases TRMT1 and TRMT1L upon neuronal activation.RNA biology · 2021Article
- Broad-range RNA modification analysis of complex biological samples using rapid C18-UPLC-MS.RNA biology · 2021Article
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11 authors.
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Abstract
Post-transcriptional chemical modifications of (t)RNA molecules are crucial in fundamental biological processes, such as translation. Despite their biological importance and accumulating evidence linking them to various human diseases, technical challenges have limited their detection and accurate quantification. Here, we present a sensitive capillary nanoflow liquid chromatography mass spectrometry (nLC-MS) pipeline for quantitative high-resolution analysis of ribonucleoside modifications from complex biological samples. We evaluated two porous graphitic carbon (PGC) materials and one end-capped C18 reference material as stationary phases for reversed-phase separation. We found that these matrices have complementing retention and separation characteristics, including the capability to separate structural isomers. PGC and C18 matrices yielded excellent signal-to-noise ratios in nLC-MS while differing in the separation capability and sensitivity for various nucleosides. This emphasizes the need for tailored LC-MS setups for optimally detecting as many nucleoside modifications as possible. Detection ranges spanning up to six orders of magnitude enable the analysis of individual ribonucleosides down to femtomol concentrations. Furthermore, normalizing the obtained signal intensities to a stable isotope labeled spike-in enabled direct comparison of ribonucleoside levels between different samples. In conclusion, capillary columns coupled to nLC-MS constitute a powerful and sensitive tool for quantitative analysis of modified ribonucleosides in complex biological samples. This setup will be invaluable for further unraveling the intriguing and multifaceted biological roles of RNA modifications.
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