ArticleParasites & vectors2018
Amino acid permease 3 (aap3) coding sequence as a target for Leishmania identification and diagnosis of leishmaniases using high resolution melting analysis.
Article in Parasites & vectors, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 26 citations in OpenAlex.
- Assessment of High-Resolution Melting Curve Analysis forPathogens (Basel, Switzerland) · 2024Article
- Accurate Identification ofJournal of arthropod-borne diseases · 2022Article
- High-resolution melting analysis identifies reservoir hosts of zoonotic Leishmania parasites in Tunisia.Parasites & vectors · 2022Article
- Two Leishmania species separation targeting the ITS-rDNA and Cyt b genes by developing and evaluating HRM- qPCR.Revista da Sociedade Brasileira de Medicina Tropical · 2022Article
- Feline Leishmaniosis: An Emerging Public Health Problem.Veterinary sciences · 2021Review
- Rapid screening ofExperimental and therapeutic medicine · 2021Article
- Developing, Modifying, and Validating a TaqMan Real-Time PCR Technique for Accurate Identification ofFrontiers in cellular and infection microbiology · 2021Article
- Laboratory Diagnosis of Cutaneous and Visceral Leishmaniasis: Current and Future Methods.Microorganisms · 2020Review
- One Health Approach to Leishmaniases: Understanding the Disease Dynamics through Diagnostic Tools.Pathogens (Basel, Switzerland) · 2020Review
- High resolution melting analysis of the 18S rRNA gene for the rapid diagnosis of bovine babesiosis.Parasites & vectors · 2019Article
- Identification of Leishmania species by high-resolution DNA dissociation in cases of American cutaneous leishmaniasis.Anais brasileiros de dermatologiaArticle
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundThe leishmaniases comprise a spectrum of clinical manifestations caused by different species of Leishmania. Identification of species is important for diagnosis, treatment and follow-up management. However, there is no gold standard for species identification. High resolution melting analysis (HRM) offers a possibility to differentiate Leishmania species without the need for processing of the PCR-product. The amino acid permease 3 (aap3) gene is an exclusive target for trypanosomatids and is conserved among Leishmania spp., thus it can be a valuable target for an HRM assay for diagnosis of the leishmaniases.
resultsThe HRM dissociation profiles of three amplicons targeting the aap3-coding region allowed the discrimination of L. (Leishmania) donovani, L. (L.) infantum, L. (L.) major, L. (L.) tropica, L. (L.) mexicana, L. (L.) amazonensis, L. (Viannia) braziliensis, L. (V.) guyanensis, L. (V.) lainsoni, L. (V.) naiffi and L. (V.) shawi using DNA from promastigote cultures. The protocol was validated with DNA samples from clinical infection in humans and a cat, naturally infected sand flies, and experimentally infected mice.
conclusionsHRM analysis using the aap3 coding sequence as target is a relatively cheap, fast and robust strategy to detect and discriminate Leishmania species from all the endemic regions worldwide. The target and method proved to be useful in clinical, field and experimental samples, thus it could be used as a tool in diagnosis as well as ecological and epidemiological studies.
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