Evidence map›Paper›PMID 30011969›Full record

ArticleInternational journal of molecular sciences2018

A Non-Enzymatic Method to Obtain a Fat Tissue Derivative Highly Enriched in Adipose Stem Cells (ASCs) from Human Lipoaspirates: Preliminary Results.

Francesco De Francesco, Silvia Mannucci, Giamaica Conti, Elena Dai Prè, Andrea Sbarbati, Michele Riccio

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed, 3 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 3 syntheses or guidelines pooled it.

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  3. Comparing mechanical and enzymatic isolation procedures to isolate adipose-derived stromal vascular fraction: A systematic review.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Francesco De FrancescoDepartment of Reconstructive Surgery and Hand Surgery (SOD Chirurgia Ricostruttiva e Chirurgia della Mano), AOU "Ospedali Riuniti", via Conca, 60126 Ancona, Italy. fran.defr@libero.it.
Silvia MannucciDepartment of Neuroscience, Biomedicine and Movement, Human Anatomy and Histology Section, University of Verona, 37134 Verona, Italy. silman82@hotmail.com.
Giamaica ContiDepartment of Neuroscience, Biomedicine and Movement, Human Anatomy and Histology Section, University of Verona, 37134 Verona, Italy. giamaica.conti@univr.it.
Elena Dai PrèDepartment of Neuroscience, Biomedicine and Movement, Human Anatomy and Histology Section, University of Verona, 37134 Verona, Italy. elena.daipre@univr.it.
Andrea SbarbatiDepartment of Neuroscience, Biomedicine and Movement, Human Anatomy and Histology Section, University of Verona, 37134 Verona, Italy. andrea.sbarbati@univr.it.
Michele RiccioDepartment of Reconstructive Surgery and Hand Surgery (SOD Chirurgia Ricostruttiva e Chirurgia della Mano), AOU "Ospedali Riuniti", via Conca, 60126 Ancona, Italy. michele.riccio@ospedaliriuniti.marche.it.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Adipose tissue possesses phenotypic gene expression characteristics that are similar to human mesenchymal stem cells (hMSCs). Nevertheless, the multilineage potential may be inhibited, and cells may not expand adequately to satisfy the requirements of Good Manufacturing Practice (cGMP). An autologous hMSC-enriched fat product would fulfil the void from a biomedical and clinical perspective. In this study, we suggest a novel mechanism using a closed system without enzymes, additives or other modifications, which will produce non-expanded, accessible material. This decentralized fat product, unlike unprocessed lipoaspirates, adequately encloses the vascular stroma with adipocytes and stromal stalks along with their vascular channels and lumina. This fat product contained hASCs and fewer hematopoietic elements such as lipoaspirates, which were digested enzymatically according to flow cytometric investigations, and molecular analysis also showed significant hASC uniformity within the cells of the stromal vascular tissue. Moreover, the fat product produced a higher quantity of hASCs similar to hMSCs in isolation with the typical characteristics of an osteogenic, chondrogenic and adipogenic lineage. Interestingly, these properties were evident in the non-enzymatic derived adipose tissue, as opposed to hASCs in isolation from the enzymatically digested lipoaspirates, suggesting that the aforementioned procedure may be an adequate alternative to regenerate and engineer tissue for the treatment of various medical conditions and promote efficient patient recovery.

Indexed as

AdipocytesAdipogenesisAdipose TissueAdultCell DifferentiationCell ProliferationCells, CulturedChondrogenesisFemaleGene ExpressionHumansLipectomyMesenchymal Stem CellsMiddle AgedOsteogenesisTissue Engineeringadipose stem cellsadipose tissueenzymatic digestionnon-enzymatic methodRigenera protocol

Identifiers

PMID30011969
PMCPMC6073668

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.