ArticlePloS one2018
The murine female intestinal microbiota does not shift throughout the estrous cycle.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 24 citations in OpenAlex.
- Sex-Dependent Microbial and Host Profiles Following Fecal Microbiota andGut and liver · 2026Article
- Effects of sexual dimorphism and estrous cycle on murineInfection and immunity · 2026Article
- Both donor and recipient sex determine behavioral and neuroimmune outcomes in a gut microbiota transfer experiment from the unpredictable chronic mild stress model of depression.Brain, behavior, & immunity - health · 2026Article
- Characterization of the gut microbiome of wildFrontiers in microbiomes · 2026Article
- Chronic variable stress leads to sex specific gut microbiome alterations in mice.Brain, behavior, & immunity - health · 2024Article
- Sex differences in a mouse model of diet-induced obesity: the role of the gut microbiome.Biology of sex differences · 2024Article
- Gut dysbiosis and age-related neurological diseases in females.Neurobiology of disease · 2022Review
- Intestinal microbial diversity in female rhesus (Frontiers in microbiology · 2022Article
- Analysis of Gut Microbiota and Metabolites in Diannan Small Ear Sows at Diestrus and Metestrus.Frontiers in microbiology · 2022Article
- Repeated Collection of Vaginal Smear Causes Stress in Mice.Noro psikiyatri arsivi · 2022Article
- Estrous cycle and ovariectomy-induced changes in visceral pain are microbiota-dependent.iScience · 2021Article
- Article
- Relationship between gut microbiota and host-metabolism: Emphasis on hormones related to reproductive function.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2021Review
- Investigation of the impact of gut microbiotas on fertility of stored sperm by types of hens.Poultry science · 2020Article
- The rodent vaginal microbiome across the estrous cycle and the effect of genital nerve electrical stimulation.PloS one · 2020Article
- Obesity during pregnancy results in maternal intestinal inflammation, placental hypoxia, and alters fetal glucose metabolism at mid-gestation.Scientific reports · 2019Article
- Comparative Analysis of Gut Microbiota Among the Male, Female and Pregnant Giant Pandas (Open life sciences · 2019Article
- Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pregnancy is accompanied by maternal physiological adaptations including metabolic, endocrine, immune, cardiovascular, skeletomuscular and neurological modifications that facilitate fetal and placental growth and development. Emerging evidence suggests that the maternal intestinal microbiota is modified over the course of healthy pregnancy. We have recently identified a maternal intestinal microbial shift within hours of conception; a shift that continued with advancing gestation. It is possible that maternal gut bacterial profiles might be associated with the known endocrine changes that accompany the female reproductive (estrous) cycle.
methodsTo determine whether the estrous cycle influenced the shifts in the maternal intestinal microbiota, time-matched fecal pellets were collected daily for 3 consecutive estrous cycles from individually housed, non-pregnant female C57BL/6J mice (n = 10) fed a control diet. Estrous stage was identified by cell type predominance in vaginal cytological samples. The corresponding fecal pellets for each estrous stage were processed for bacterial 16S rRNA sequencing of the variable 3 (V3) region.
resultsEstrous cycle stage accounted for a very small and not statistically significant proportion of the variation in the fecal microbiota according to PERMANOVA testing performed on Bray-Curtis dissimilarity scores. These values displayed no significant clustering of fecal microbial communities by estrous stage.
conclusionThe estrous cycle does not result in any significant shift in the intestinal microbial community in the reproductively mature, regularly cycling female mouse.
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