ArticleNucleic acids research2018
Fluorescence spectroscopic detection and measurement of single telomere molecules.
Article in Nucleic acids research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- High-throughput single telomere analysis using DNA microarray and fluorescent in situ hybridization.Nucleic acids research · 2024Article
- Telomere Dynamics in Livestock.Biology · 2023Review
- High-throughput telomere length measurement at nucleotide resolution using the PacBio high fidelity sequencing platform.Nature communications · 2023Article
- Payload distribution and capacity of mRNA lipid nanoparticles.Nature communications · 2022Article
- The advancement of telomere quantification methods.Molecular biology reports · 2021Review
- Massively parallel single-molecule telomere length measurement with digital real-time PCR.Science advances · 2020Article
- Electrode-Free Concentration and Recovery of DNA at Physiologically Relevant Ionic Concentrations.Analytical chemistry · 2020Article
- Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Telomeres are the end-caps of chromosomes that serve to protect the integrity of the genome. Below certain critical lengths, the telomeres can no longer fulfill their protective function, and chromosomal instability ensues. Telomeres shorten during normal cell division due to the end replication problem and are implicated in the development of various aging-associated diseases, including cancer. Telomere length has the potential to serve as a useful biomarker in the field of aging and cancer. However, existing methods of telomere measurement are either too laborious, unable to provide absolute measurement of individual telomere lengths, or limited to certain chromosomes or cell types. Here, we describe an easy single-molecule, fluorescence spectroscopic method for measuring the length of telomeres that permits the profiling of absolute telomere lengths in any DNA sample. We have demonstrated the accurate detection of telomeres as short as 100 bp using cloned telomere standards, and have profiled telomere lengths in human cancer cell lines and primary cells. Since this method allows direct comparison between samples, it could greatly improve the clinical utility of telomere biomarkers.
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Registered trials
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