ArticleNature biotechnology2018
Multiplexed identification, quantification and genotyping of infectious agents using a semiconductor biochip.
Article in Nature biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 73 citations in OpenAlex.
- Severe paediatric scrub typhus with complications: a case report and literature review.BMC pediatrics · 2025Review
- ISFET Biosensor with Loop-Mediated Isothermal Amplification for Electronic Rapid Detection of Mycoplasma Pneumoniae.Sensors (Basel, Switzerland) · 2025Article
- Article
- Exploring the Utility of Multiplex Infectious Disease Panel Testing for Diagnosis of Infection in Different Body Sites: A Joint Report of the Association for Molecular Pathology, American Society for Microbiology, Infectious Diseases Society of America, and Pan American Society for Clinical Virology.The Journal of molecular diagnostics : JMD · 2023Review
- Semiautomated Electrochemical Melting Curve Analysis Device for the Detection of an Osteoporosis Associated Single Nucleotide Polymorphism in Blood.Analytical chemistry · 2023Article
- Recent Advances in Metaphotonic Biosensors.Biosensors · 2023Review
- Advances in point-of-care genetic testing for personalized medicine applications.Biomicrofluidics · 2023Review
- Calibration-free, high-precision, and robust terahertz ultrafast metasurfaces for monitoring gastric cancers.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Ultrasensitive Detection of Biomarkers in a Color-Switchable Microcavity-Reactor Laser.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Low-Cost Platform for Multiplexed Electrochemical Melting Curve Analysis.ACS measurement science au · 2022Article
- Metagenomics-enabled microbial surveillance.Nature microbiology · 2022Review
- Highly multiplex PCR assays by coupling the 5'-flap endonuclease activity ofProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Improved targeting of the 16S rDNA nanopore sequencing method enables rapid pathogen identification in bacterial pneumonia in children.Frontiers in cellular and infection microbiology · 2022Article
- Integration of Ultra-Low Volume Pneumatic Microfluidics with a Three-Dimensional Electrode Network for On-Chip Biochemical Sensing.Micromachines · 2021Article
- An ISFET Microarray Sensor System for Detecting the DNA Base Pairing.Micromachines · 2021Article
- Epidemiological and clinical characteristics of suspected COVID-19 patients in the isolation ward in Guangzhou, China: a cohort study.Annals of translational medicine · 2021Article
- Prospective Evaluation of a Rapid Clinical Metagenomics Test for Bacterial Pneumonia.Frontiers in cellular and infection microbiology · 2021Article
- High resolution biosensor to test the capping level and integrity of mRNAs.Nucleic acids research · 2020Article
- Human Organs-on-Chips for Virology.Trends in microbiology · 2020Review
- Article
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Authors and funding
34 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The emergence of pathogens resistant to existing antimicrobial drugs is a growing worldwide health crisis that threatens a return to the pre-antibiotic era. To decrease the overuse of antibiotics, molecular diagnostics systems are needed that can rapidly identify pathogens in a clinical sample and determine the presence of mutations that confer drug resistance at the point of care. We developed a fully integrated, miniaturized semiconductor biochip and closed-tube detection chemistry that performs multiplex nucleic acid amplification and sequence analysis. The approach had a high dynamic range of quantification of microbial load and was able to perform comprehensive mutation analysis on up to 1,000 sequences or strands simultaneously in <2 h. We detected and quantified multiple DNA and RNA respiratory viruses in clinical samples with complete concordance to a commercially available test. We also identified 54 drug-resistance-associated mutations that were present in six genes of Mycobacterium tuberculosis, all of which were confirmed by next-generation sequencing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.