ArticleJournal of visualized experiments : JoVE2018
High-throughput DNA Extraction and Genotyping of 3dpf Zebrafish Larvae by Fin Clipping.
Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
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- A rapid and cost-effective Chelex-100 resin-based method for genomic DNA extraction from adult zebrafish tissues.PloS one · 2026Article
- Developmental regulation of intestinalbioRxiv : the preprint server for biology · 2025Article
- A simple and rapid shaking-based assay to genotype live, early developmental stage zebrafish embryos.Laboratory animals · 2025Article
- Pain management in zebrafish : Report from a FELASA Working Group.Laboratory animals · 2024Review
- Fast, precise and cloning-free knock-in of reporter sequences in vivo with high efficiency.Development (Cambridge, England) · 2023Article
- Article
- A minimally invasive fin scratching protocol for fast genotyping and early selection of zebrafish embryos.Scientific reports · 2022Article
- Adar-mediated A-to-I editing is required for embryonic patterning and innate immune response regulation in zebrafish.Nature communications · 2022Article
- Robo2 Drives Target-Selective Peripheral Nerve Regeneration in Response to Glia-Derived Signals.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- Zebrafish, Medaka and Turquoise Killifish for Understanding Human Neurodegenerative/Neurodevelopmental Disorders.International journal of molecular sciences · 2022Review
- Genomic evidence supports the introgression between two sympatric stickleback species inhabiting the White Sea basin.Heliyon · 2021Article
- PLPHP deficiency: clinical, genetic, biochemical, and mechanistic insights.Brain : a journal of neurology · 2019Article
- Characterization of the zebrafish gabra1Differentiation; research in biological diversityArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
Zebrafish (Danio rerio) possess orthologues for 84% of the genes known to be associated with human diseases. In addition, these animals have a short generation time, are easy to handle, display a high reproductive rate, low cost, and are easily amenable to genetic manipulations by microinjection of DNA in embryos. Recent advances in gene editing tools are enabling precise introduction of mutations and transgenes in zebrafish. Disease modeling in zebrafish often leads to larval phenotypes and early death which can be challenging to interpret if genotypes are unknown. This early identification of genotypes is also needed in experiments requiring sample pooling, such as in gene expression or mass spectrometry studies. However, extensive genotypic screening is limited by traditional methods, which in most labs are performed only on adult zebrafish or in postmortem larvae. We addressed this problem by adapting a method for the isolation of PCR-ready genomic DNA from live zebrafish larvae that can be achieved as early as 72 h post-fertilization (hpf). This time and cost-effective technique, improved from a previously published genotyping protocol, allows the identification of genotypes from microscopic fin biopsies. The fins quickly regenerate as the larvae develop. Researchers are then able to select and raise the desired genotypes to adulthood by utilizing this high-throughput PCR-based genotyping procedure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.