Evidence map›Paper›PMID 30009850›Full record

ArticleJournal of virological methods2018

Evaluation of an incubation instrument-free reverse transcription recombinase polymerase amplification assay for rapid and point-of-need detection of canine distemper virus.

Jianchang Wang, Jinfeng Wang, Ruiwen Li, Ruihan Shi, Libing Liu, Wanzhe Yuan

Abstract readEvaluation Study
In one paragraph

Article in Journal of virological methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.6field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 19 citations in OpenAlex.

  1. Article
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  4. Review
  5. Article
  6. Rapid, Simple, and Highly Specific Detection ofFrontiers in cellular and infection microbiology · 2022
    Article
  7. Rapid Detection ofFrontiers in cellular and infection microbiology · 2022
    Article
  8. Article
  9. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Jianchang WangCenter of Inspection and Quarantine, Hebei Entry-Exit Inspection and Quarantine Bureau, Shijiazhuang 050051, China; Hebei Academy of Science and Technology for Inspection and Quarantine, Shijiazhuang 050051, China.
Jinfeng WangCenter of Inspection and Quarantine, Hebei Entry-Exit Inspection and Quarantine Bureau, Shijiazhuang 050051, China; Hebei Academy of Science and Technology for Inspection and Quarantine, Shijiazhuang 050051, China.
Ruiwen LiCollege of Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, China.
Ruihan ShiCenter of Inspection and Quarantine, Hebei Entry-Exit Inspection and Quarantine Bureau, Shijiazhuang 050051, China; Hebei Academy of Science and Technology for Inspection and Quarantine, Shijiazhuang 050051, China.
Libing LiuCenter of Inspection and Quarantine, Hebei Entry-Exit Inspection and Quarantine Bureau, Shijiazhuang 050051, China; Hebei Academy of Science and Technology for Inspection and Quarantine, Shijiazhuang 050051, China.
Wanzhe YuanCollege of Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, China. Electronic address: yuanwanzhe2015@126.com.
Hebei Food Inspection and Research Institute · CNHebei Agricultural University · CNEntry Exit Inspection and Quarantine Bureau · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Canine distemper, caused by Canine distemper virus (CDV), is a highly contagious and fatal systemic disease in free-living and captive carnivores worldwide. Accurate, rapid and simple detection of CDV is critical to improve disease management and prevent outbreaks. In this study, a visible and incubation instrument-free reverse-transcription recombinase polymerase amplification assay combined with lateral flow strip (LFS RT-RPA) was developed to detect CDV using primers and lateral flow (LF) probe specific for the nucleocapsid (N) protein gene. The CDV LFS RT-RPA assay was performed in a closed fist using body heat for 15 min, and the products were visible to the naked eyes on the LFS within 5 min. The assay could detect CDV, and there was no cross-reaction with the other viruses tested. Using the in vitro transcribed CDV RNA as template, the analytical sensitivity was 9.4 × 10

Indexed as

Nucleic Acid Amplification TechniquesAnimalsDistemperDistemper Virus, CanineDogsNucleocapsid ProteinsRecombinasesReproducibility of ResultsReverse TranscriptionRNA, ViralSensitivity and SpecificityNucleocapsid ProteinsRecombinasesRNA, ViralCDVLF probeLFSN geneRT-RPA

Identifiers

PMID30009850
PMCPMC7113680
OpenAlexW2884646676

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.