Evidence map›Paper›PMID 30007855›Full record

ArticleBlood cells, molecules & diseases2018

In-depth phenotypic characterization of reticulocyte maturation using mass cytometry.

Richard Thomson-Luque, Chengqi Wang, Francis B Ntumngia, Shulin Xu, Karoly Szekeres, Amy Conway, Swamy Rakesh Adapa, Samantha J Barnes, John H Adams, Rays H Y Jiang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Blood cells, molecules & diseases, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed.

  1. Article
  2. Targeting anemia-induced CD71Infection and immunity · 2025
    Article
  3. Article
  4. Article
  5. Review
  6. Article
  7. Article
  8. Article
  9. Reticulocyte Maturation.Membranes · 2022
    Review
  10. Article
  11. Review
  12. Review
  13. Review
  14. Article
  15. Home Sweet Home:Frontiers in cellular and infection microbiology · 2021
    Review
  16. A gut feeling about stroke reveals gut-brain axis' active role in homeostasis and dysbiosis.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2020
    Article
  17. Review
  18. Article
  19. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Richard Thomson-LuqueCenter for Global Health and Infectious Diseases Research, College of Public Health, University of South Florida, Tampa, USA.
Chengqi WangCenter for Global Health and Infectious Diseases Research, College of Public Health, University of South Florida, Tampa, USA.
Francis B NtumngiaCenter for Global Health and Infectious Diseases Research, College of Public Health, University of South Florida, Tampa, USA.
Shulin XuCenter for Global Health and Infectious Diseases Research, College of Public Health, University of South Florida, Tampa, USA.
Karoly SzekeresDepartment of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, USA.
Amy ConwayCenter for Global Health and Infectious Diseases Research, College of Public Health, University of South Florida, Tampa, USA.
Swamy Rakesh AdapaCenter for Global Health and Infectious Diseases Research, College of Public Health, University of South Florida, Tampa, USA.
Samantha J BarnesCenter for Global Health and Infectious Diseases Research, College of Public Health, University of South Florida, Tampa, USA.
John H AdamsCenter for Global Health and Infectious Diseases Research, College of Public Health, University of South Florida, Tampa, USA. Electronic address: jadams3@health.usf.edu.
Rays H Y JiangCenter for Global Health and Infectious Diseases Research, College of Public Health, University of South Florida, Tampa, USA. Electronic address: jiang2@health.usf.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Progress towards an in-depth understanding of the final steps of the erythroid lineage development is paramount for many hematological diseases. We have characterized the final stages of reticulocyte maturation from bone marrow to peripheral blood using for the first time single-cell Mass Cytometry (CyTOF). We were able to measure the expression of 31 surface markers within a single red blood cell (RBC). We demonstrate the validity of CyTOF for RBC phenotyping by confirming the progressive reduction of transferrin receptor 1 (CD71) during reticulocyte maturation to mature RBC. We highlight the high-dimensional nature of mass cytometry data by correlating the expression of multiple proteins on individual RBCs. We further describe a more drastic reduction pattern for a component of the alpha4/beta1 integrin CD49d at the very early steps of reticulocyte maturation in bone marrow and directly linked with the mitochondria remnants clearance pattern. The enhanced and accurate RBC phenotyping potential of CyTOF described herein could be beneficial to decipher RBC preferences, as well as still not well understood receptor-ligand interaction of some hemotropic parasites such as the malaria causing agent Plasmodium vivax.

Indexed as

AnimalsAntigens, CDBiomarkersCell DifferentiationCell LineageCytological TechniquesErythrocytesHumansImmunophenotypingIntegrin alpha4Receptors, TransferrinReticulocytesSingle-Cell AnalysisAntigens, CDBiomarkersCD71 antigenIntegrin alpha4Receptors, TransferrinCyTOFMass cytometryMaturationRed blood cellReticulocyte

Identifiers

PMID30007855
PMCPMC6097872

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.