ArticleNucleic acids research2018
VpsR and cyclic di-GMP together drive transcription initiation to activate biofilm formation in Vibrio cholerae.
Article in Nucleic acids research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 50 citations in OpenAlex.
- Article
- Environmental regulation of virulence in Vibrio cholerae: integrating multi-omics, predictive models, and comparative insights across emerging Vibrio species.Gut pathogens · 2026Review
- RpoN mediates biofilm formation by directly controllingBiofilm · 2025Article
- Article
- Negative feedback of cyclic di-GMP levels optimizes switching between sessile and motile lifestyles inbioRxiv : the preprint server for biology · 2024Article
- Review
- Article
- pGpG-signaling regulates virulence and global transcriptomic targets inbioRxiv : the preprint server for biology · 2024Article
- TheJournal of bacteriology · 2023Article
- ThebioRxiv : the preprint server for biology · 2023Article
- New Insights into Vibrio cholerae Biofilms from Molecular Biophysics to Microbial Ecology.Advances in experimental medicine and biology · 2023Article
- Dual Regulatory Role Exerted by Cyclic Dimeric GMP To Control FsnR-Mediated Bacterial Swimming.mBio · 2022Article
- Quorum-sensing control of matrix protein production drives fractal wrinkling and interfacial localization of Vibrio cholerae pellicles.Nature communications · 2022Article
- The Vibrio cholerae master regulator for the activation of biofilm biogenesis genes, VpsR, senses both cyclic di-GMP and phosphate.Nucleic acids research · 2022Article
- Persulfide-Responsive Transcription Factor SqrR Regulates Gene Transfer and Biofilm Formation via the Metabolic Modulation of Cyclic di-GMP inMicroorganisms · 2022Article
- Roles of the second messenger c-di-GMP in bacteria: Focusing on the topics of flagellar regulation and Vibrio spp.Genes to cells : devoted to molecular & cellular mechanisms · 2022Review
- Quantitative input-output dynamics of a c-di-GMP signal transduction cascade in Vibrio cholerae.PLoS biology · 2022Article
- Genome-wide mapping of Vibrio cholerae VpsT binding identifies a mechanism for c-di-GMP homeostasis.Nucleic acids research · 2022Article
- Impact of Gene Repression on Biofilm Formation ofFrontiers in microbiology · 2022Article
- A phage-encoded nucleoid associated protein compacts both host and phage DNA and derepresses H-NS silencing.Nucleic acids research · 2021Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The small molecule cyclic di-GMP (c-di-GMP) is known to affect bacterial gene expression in myriad ways. In Vibrio cholerae in vivo, the presence of c-di-GMP together with the response regulator VpsR results in transcription from PvpsL, a promoter of biofilm biosynthesis genes. VpsR shares homology with enhancer binding proteins that activate σ54-RNA polymerase (RNAP), but it lacks conserved residues needed to bind to σ54-RNAP and to hydrolyze adenosine triphosphate, and PvpsL transcription does not require σ54 in vivo. Consequently, the mechanism of this activation has not been clear. Using an in vitro transcription system, we demonstrate activation of PvspL in the presence of VpsR, c-di-GMP and σ70-RNAP. c-di-GMP does not significantly change the affinity of VpsR for PvpsL DNA or the DNase I footprint of VpsR on the DNA, and it is not required for VpsR to dimerize. However, DNase I and KMnO4 footprints reveal that the σ70-RNAP/VpsR/c-di-GMP complex on PvpsL adopts a different conformation from that formed by σ70-RNAP alone, with c-di-GMP or with VpsR. Our results suggest that c-di-GMP is required for VpsR to generate the specific protein-DNA architecture needed for activated transcription, a previously unrecognized role for c-di-GMP in gene expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.