Evidence map›Paper›PMID 30006473›Full record

ArticleBioscience reports2018

Controlling the regioselectivity and stereospecificity of FAD-dependent polyamine oxidases with the use of amine-attached guide molecules as conformational modulators.

Tuomo A Keinänen, Nikolay Grigorenko, Alex R Khomutov, Qingqiu Huang, Anne Uimari, Leena Alhonen, Mervi T Hyvönen, Jouko Vepsäläinen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioscience reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 3 countries.

Tuomo A KeinänenSchool of Pharmacy, Biocenter Kuopio, University of Eastern Finland, Kuopio Campus, P.O. Box 1627, Kuopio FI-70211, Finland tuomo.keinanen@uef.fi.
Nikolay GrigorenkoBASF Schweiz AG, Dispersions and Pigments Division, Klybeckstrasse 141, P.O. Box CH 4002, Basel, Switzerland.
Alex R KhomutovEngelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov St 32, Moscow 119991, Russia.
Qingqiu HuangMacCHESS at the Cornell High Energy Synchrotron Source, Cornell University Ithaca, NY 14853-8001, U.S.A.
Anne UimariNatural Resources Institute Finland, Natural Resources Division, Neulaniementie 5, Kuopio FI-70210, Finland.
Leena AlhonenSchool of Pharmacy, Biocenter Kuopio, University of Eastern Finland, Kuopio Campus, P.O. Box 1627, Kuopio FI-70211, Finland.
Mervi T HyvönenSchool of Pharmacy, Biocenter Kuopio, University of Eastern Finland, Kuopio Campus, P.O. Box 1627, Kuopio FI-70211, Finland.
Jouko VepsäläinenSchool of Pharmacy, Biocenter Kuopio, University of Eastern Finland, Kuopio Campus, P.O. Box 1627, Kuopio FI-70211, Finland.
University of Eastern Finland · FIBASF (Switzerland) · CHEngelhardt Institute of Molecular Biology · RUNatural Resources Institute Finland · FI

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Enzymes generally display strict stereospecificity and regioselectivity for their substrates. Here by using FAD-dependent human acetylpolyamine oxidase (APAO), human spermine (Spm) oxidase (SMOX) and yeast polyamine oxidase (Fms1), we demonstrate that these fundamental properties of the enzymes may be regulated using simple guide molecules, being either covalently attached to polyamines or used as a supplement to the substrate mixtures. APAO, which naturally metabolizes achiral

Indexed as

AcylationAlkylationAnimalsDrug DiscoveryFlavin-Adenine DinucleotideHumansLigandsOxidoreductases Acting on CH-NH Group DonorsPolyamine OxidaseRats, WistarSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSpermidineSpermineStereoisomerismSubstrate Specificity1-methylspermidineFlavin-Adenine DinucleotideFMS1 protein, S cerevisiaeLigandsOxidoreductases Acting on CH-NH Group DonorsPolyamine OxidaseSaccharomyces cerevisiae ProteinsSpermidineSpermineamine oxidoreductasebiocatalysischiralitydrug discovery and designflavins

Identifiers

PMID30006473
PMCPMC6131205
OpenAlexW2884091820

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.