ReviewExpert review of proteomics2018
Proteomics and pulse azidohomoalanine labeling of newly synthesized proteins: what are the potential applications?
Review in Expert review of proteomics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed.
- Rank Normalization Enables Cross Pathway Comparison in Degradation Resolved Proteome Turnover Analysis.bioRxiv : the preprint server for biology · 2026Article
- Incorporation of azide groups into an engineered Fn3 scaffold protein for site-specific, bioorthogonal conjugation.Protein engineering, design & selection : PEDS · 2026Article
- An Enigma of N-termini dependent protein degradation.Biochemistry and biophysics reports · 2025Article
- Novel Strategy for Acquiring Metabolically-Tagged Nascent Extracellular Vesicles: Implications for Identifying Surface Protein Markers of Extracellular Vesicles From Neuroblastoma Cells Cultured With Native Serum.Journal of extracellular vesicles · 2025Article
- Residue-Specific Incorporation of Noncanonical Amino Acids in Auxotrophic Hosts:Chemical reviews · 2025Review
- Proteomics of the heart.Physiological reviews · 2024Review
- Article
- An integrated workflow for quantitative analysis of the newly synthesized proteome.Nature communications · 2023Article
- Recent advancements in mass spectrometry-based tools to investigate newly synthesized proteins.Current opinion in chemical biology · 2022Review
- Revealing functional insights into ER proteostasis through proteomics and interactomics.Experimental cell research · 2021Review
- SPAAC Pulse-Chase: A Novel Click Chemistry-Based Method to Determine the Half-Life of Cellular Proteins.Frontiers in cell and developmental biology · 2021Article
- Fishing for newly synthesized proteins with phosphonate-handles.Nature communications · 2020Article
- Common principles and best practices for engineering microbiomes.Nature reviews. Microbiology · 2019Review
- Article
- Physical and Molecular Landscapes of Mouse Glioma Extracellular Vesicles Define Heterogeneity.Cell reports · 2019Article
- Mass spectrometry-based methods for investigating the dynamics and organization of the surfaceome: exploring potential clinical implications.Expert review of proteomicsReview
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
introductionMeasuring the immediate changes in cells that arise from changing environmental conditions is crucial to understanding the underlying mechanisms involved. These changes can be measured with metabolic stable isotope fully labeled proteomes, but requires looking for changes in the midst of a large background. In addition, labeling efficiency can be an issue in primary and fully differentiated cells. Area covered: Azidohomoalanine (AHA), an analog of methionine, can be accepted by cellular translational machinery and incorporated into newly synthesized proteins (NSPs). AHA-NSPs can be coupled to biotin via CuAAC-mediated click-chemistry and enriched using avidin-based affinity purification. Thus, AHA-containing proteins or peptides can be enriched and efficiently separated from the whole proteome. In this review, we describe the development of mass spectrometry (MS) based AHA strategies and discuss their potential to measure proteins involved in immune response, secretome, gut microbiome, and proteostasis as well as their potential for clinical uses. Expert commentary: AHA strategies have been used to identify synthesis activity and to compare two biological conditions in various biological model organisms. In combination with instrument development, improved sample preparation and fractionation strategies, MS-based AHA strategies have the potential for broad application, and the methods should translate into clinical use.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.