ArticleBioconjugate chemistry2018
Characterizing the Surface Coverage of Protein-Gold Nanoparticle Bioconjugates.
Article in Bioconjugate chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Nanosystems Containing Proteins: From Fundamentals to Applications.Chemistry, an Asian journal · 2026Review
- Article
- Cancer Cell Targeting Via Selective Transferrin Receptor Labeling Using Protein-Derived Carbon Dots.ACS omega · 2024Article
- Anti-Tumor Activity of Novel Nimotuzumab-Functionalized Gold Nanoparticles as a Potential Immunotherapeutic Agent against Skin and Lung Cancers.Journal of functional biomaterials · 2023Article
- Structural Transitions of Papain-like Cysteine Proteases: Implications for Sensor Development.Biomimetics (Basel, Switzerland) · 2023Article
- Metal-based nanomaterials and nanocomposites as promising frontier in cancer chemotherapy.MedComm · 2023Review
- Amplified EQCM-D detection of extracellular vesicles using 2D gold nanostructured arrays fabricated by block copolymer self-assembly.Nanoscale horizons · 2023Article
- Evaluating methods to create protein functionalized catanionic vesicles.Soft matter · 2023Article
- Measuring the refractive index and sub-nanometre surface functionalisation of nanoparticles in suspension.Nanoscale · 2022Article
- Designing Functional Bionanoconstructs for EffectiveBioconjugate chemistry · 2022Review
- Spectral image contrast-based flow digital nanoplasmon-metry for ultrasensitive antibody detection.Journal of nanobiotechnology · 2022Article
- Analytical methods of antibody surface coverage and orientation on bio-functionalized magnetic beads: application to immunocapture of TNF-α.Analytical and bioanalytical chemistry · 2021Article
- Effect of gold nanoparticles on the structure and neuroprotective function of protein L-isoaspartyl methyltransferase (PIMT).Scientific reports · 2021Article
- Article
- Rapid and Easy-to-Use Method for Accurate Characterization of Target Binding and Kinetics of Magnetic Particle Bioconjugates for Biosensing.Sensors (Basel, Switzerland) · 2021Article
- Acceleration of catalysis in dihydrofolate reductase by transient, site-specific photothermal excitation.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- A click-based modular approach to introduction of peroxides onto molecules and nanostructures.RSC advances · 2020Article
- Measuring the Accessible Surface Area within the Nanoparticle Corona Using Molecular Probe Adsorption.Nano letters · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors.
Funding
Abstract
Functional enzyme-nanoparticle bioconjugates are increasingly important in biomedical and biotechnology applications such as drug delivery and biosensing. Optimization of the function of such bioconjugates requires careful control and characterization of their structures and activity, but current methods are inadequate for this purpose. A key shortcoming of existing approaches is the lack of an accurate method for quantitating protein content of bioconjugates for low (monolayer) surface coverages. In this study, an integrated characterization methodology for protein-gold nanoparticle (AuNP) bioconjugates is developed, with a focus on site-specific attachment and surface coverage of protein on AuNPs. Single-cysteine-containing mutants of dihydrofolate reductase are covalently attached to AuNPs with diameters of 5, 15, and 30 nm, providing a range of surface curvature. Site-specific attachment to different regions of the protein surface is investigated, including attachment to a flexible loop versus a rigid α helix. Characterization methods include SDS-PAGE, UV-vis spectrophotometry, dynamic light scattering, and a novel fluorescence-based method for accurate determination of low protein concentration on AuNPs. An accurate determination of both protein and AuNP concentration in conjugate samples allows for the calculation of the surface coverage. We find that surface coverage is related to the surface curvature of the AuNP, with a higher surface coverage observed for higher surface curvature. The combination of these characterization methods is important for understanding the functionality of protein-AuNP bioconjugates, particularly enzyme activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.