ArticleJournal of cancer prevention2018
Mechanical Alteration Associated With Chemotherapeutic Resistance of Breast Cancer Cells.
Article in Journal of cancer prevention, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 13 citations in OpenAlex.
- Deformability screening identifies NUDT5 as a mediator of cellular mechanobiology.bioRxiv : the preprint server for biology · 2026Article
- Transformative insights in breast cancer: review of atomic force microscopy applications.Discover oncology · 2025Review
- Particle uptake in cancer cells can predict malignancy and drug resistance using machine learning.Science advances · 2024Article
- Article
- A naphthalimide-based peptide conjugate for concurrent imaging and apoptosis induction in cancer cells by utilizing endogenous hydrogen sulfide.Chemical science · 2021Article
- Biomechanical Properties of Cancer Cells.Cells · 2021Review
- DNA damage alters nuclear mechanics through chromatin reorganization.Nucleic acids research · 2021Article
- Enrichment of CD44 in Exosomes From Breast Cancer Cells Treated With Doxorubicin Promotes Chemoresistance.Frontiers in oncology · 2020Article
- Article
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe mechanical deformability of cancer cells has attracted particular attention as an emerging biomarker for the prediction of anti-cancer drug sensitivity. Nevertheless, it has not been possible to establish a general rubric for the identification of drug susceptibility in breast cancer cells from a mechanical perspective. In the present study, we investigated the mechanical alteration associated with resistance to adjuvant therapy in breast cancer cells.
methodsWe performed an 'atomic force microscopy (AFM)-based nanomechanical study' on 'drug-sensitive (MCF-7)' and 'drug-resistant (MCF-7/ADR)' breast cancer cells. We also conducted cell viability tests to evaluate the difference in doxorubicin responsiveness between two breast cancer cell lines. We carried out a wound closure experiment to investigate the motility changes associated with chemotherapeutic resistance. To elucidate the changes in molecular alteration that accompany chemotherapeutic resistance, we investigated the expression of vinculin and integrin-linked kinase-1-which are proteins involved in substrate adhesion and the actin cytoskeleton-using Western blotting analysis.
resultsA MTT assay confirmed that the dose-dependent efficacy of doxorubicin was reduced in MCF-7/ADR cells compared to that in MCF-7 cells. The wound assay revealed enhanced two-dimensional motility in the MCF-7/ADR cells. The AFM mechanical assay showed evidence that the drug-resistant breast cancer cells exhibited a significant decrease in mechanical deformability compared to their drug-sensitive counterparts. The mechanical alteration in the MCF-7/ADR cells was accompanied by upregulated vinculin expression.
conclusionsThe obtained results manifestly showed that the altered mechanical signatures-including mechanical deformability and motility-were closely related with drug resistance in the breast cancer cells. We believe that this investigation has improved our understanding of the chemotherapeutic susceptibility of breast cancer cells.
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