Evidence map›Paper›PMID 30002410›Full record

ArticleScientific reports2018

Authentication of Garcinia fruits and food supplements using DNA barcoding and NMR spectroscopy.

Gopalakrishnan Saroja Seethapathy, Margey Tadesse, Santhosh Kumar J Urumarudappa, Srikanth V Gunaga, Ramesh Vasudeva, Karl Egil Malterud, Ramanan Uma Shaanker, Hugo J de Boer, Gudasalamani Ravikanth, Helle Wangensteen

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In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
6.8field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 69 citations in OpenAlex.

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  14. The efficacy of machine learning algorithm for raw drug authentication inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2021
    Article
  15. Maize specialized metabolome networks reveal organ-preferential mixed glycosides.Computational and structural biotechnology journal · 2021
    Article
  16. Review
  17. Review
  18. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Gopalakrishnan Saroja SeethapathyDepartment of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, 0316, Oslo, Norway.ORCID 0000-0002-1650-5199
Margey TadesseDepartment of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, 0316, Oslo, Norway.
Santhosh Kumar J UrumarudappaDepartment of Crop Physiology, School of Ecology and Conservation, University of Agricultural Sciences, Gandhi Krishi Vigyan Kendra, Bangalore, 560065, India.ORCID 0000-0002-8509-0089
Srikanth V GunagaDepartment of Forest Biology, College of Forestry, University of Agricultural Sciences, Sirsi, 581401, India.
Ramesh VasudevaDepartment of Forest Biology, College of Forestry, University of Agricultural Sciences, Sirsi, 581401, India.
Karl Egil MalterudDepartment of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, 0316, Oslo, Norway.
Ramanan Uma ShaankerAshoka Trust for Research in Ecology and the Environment (ATREE), Royal Enclave, Srirampura, Jakkur Post, Bangalore, 560064, India.
Hugo J de BoerNatural History Museum, University of Oslo, P.O. Box 1172, 0318, Oslo, Norway.
Gudasalamani RavikanthAshoka Trust for Research in Ecology and the Environment (ATREE), Royal Enclave, Srirampura, Jakkur Post, Bangalore, 560064, India. gravikanth@atree.org.ORCID 0000-0002-9399-8580
Helle WangensteenDepartment of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, 0316, Oslo, Norway. helle.wangensteen@farmasi.uio.no.
University of Oslo · NOUniversity of Agricultural Sciences, Bangalore · INAshoka Trust for Research in Ecology and the Environment · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Garcinia L. (Clusiaceae) fruits are a rich source of (-)-hydroxycitric acid, and this has gained considerable attention as an anti-obesity agent and a popular weight loss food supplement. In this study, we assessed adulteration of morphologically similar samples of Garcinia using DNA barcoding, and used NMR to quantify the content of (-)-hydroxycitric acid and (-)-hydroxycitric acid lactone in raw herbal drugs and Garcinia food supplements. DNA barcoding revealed that mostly G. gummi-gutta (previously known as G. cambogia) and G. indica were traded in Indian herbal markets, and there was no adulteration. The content of (-)-hydroxycitric acid and (-)-hydroxycitric acid lactone in the two species varied from 1.7% to 16.3%, and 3.5% to 20.7% respectively. Analysis of ten Garcinia food supplements revealed a large variation in the content of (-)-hydroxycitric acid, from 29 mg (4.6%) to 289 mg (50.6%) content per capsule or tablet. Only one product contained quantifiable amounts of (-)-hydroxycitric acid lactone. Furthermore the study demonstrates that DNA barcoding and NMR could be effectively used as a regulatory tool to authenticate Garcinia fruit rinds and food supplements.

Indexed as

Anti-Obesity AgentsChromatography, High Pressure LiquidCitratesDietary SupplementsDNA Barcoding, TaxonomicDrug ContaminationFood ContaminationFruitGarciniaIndiaMagnetic Resonance SpectroscopyAnti-Obesity AgentsCitrateshydroxycitric acid

Identifiers

PMID30002410
PMCPMC6043575
OpenAlexW2829706758

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.