Evidence map›Paper›PMID 30002272›Full record

ReviewJournal of biosciences2018

Fluorescence spectroscopy for revealing mechanisms in biology: Strengths and pitfalls.

G Krishnamoorthy

Abstract readReview
PubMed
In one paragraph

Review in Journal of biosciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
  2. Article
  3. Bacterial Luciferases fromInternational journal of molecular sciences · 2021
    Article
  4. Article
  5. Article
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author.

G KrishnamoorthyDepartment of Biotechnology, Anna University, Chennai 600 025, India, gk@tifr.res.in.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This article describes the basic principles of steady-state and time-resolved fluorescence. The formal equivalence of the two methodologies is described first, followed by the extra advantages of time-resolved methods in revealing population heterogeneity in complex systems encountered in biology. Several examples from the author's work are described in support of the above contention. Finally, several misinterpretations and pitfalls in the interpretation of fluorescence data and their remedy are described.

Indexed as

Bacillus amyloliquefaciensBacterial ProteinsBenzopyransDNA-Binding ProteinsFluorescence PolarizationFluorescence Recovery After PhotobleachingFluorescent DyesHydrogen-Ion ConcentrationMembrane PotentialsNaphtholsPlant ProteinsRanunculaceaeRhodaminesSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSpectrometry, FluorescenceBacterial Proteinsbarstar protein, Bacillus amyloliquefaciensBenzopyransDNA-Binding ProteinsFluorescent DyesFOB1 protein, S cerevisiaemonellin protein, Dioscoreophyllum cumminsiiNaphtholsPlant ProteinsRhodaminesSaccharomyces cerevisiae ProteinsseminaphthorhodaminefluorideTryptophan

Identifiers

PMID30002272

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.