ArticleCell proliferation2018
Effect of microgravity on proliferation and differentiation of embryonic stem cells in an automated culturing system during the TZ-1 space mission.
Article in Cell proliferation, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 51 citations in OpenAlex.
- Spaceflight multi-omics reveals vulnerabilities of human germ cell development.Science advances · 2026Article
- Simulated microgravity alters sperm navigation, fertilization and embryo development in mammals.Communications biology · 2026Article
- Advances and applications of organ-on-a-chip technology.Cell reports methods · 2026Review
- Neutral Buoyancy as a Simple Approach to Simulated Microgravity.Tissue engineering and regenerative medicine · 2026Article
- Stem cells in space: microgravity effects on stem cell fate and implications for regenerative medicine.NPJ microgravity · 2025Review
- A biological and ethical assessment of whether humans could or should reproduce in space.NPJ microgravity · 2025Review
- Biomanufacturing in low Earth orbit: A paradigm shift.Stem cell reports · 2025Review
- Oxidative Damage Under Microgravity Conditions: Response Mechanisms, Monitoring Methods and Countermeasures on Somatic and Germ Cells.International journal of molecular sciences · 2025Review
- Surface tension enables induced pluripotent stem cell culture in commercially available hardware during spaceflight.NPJ microgravity · 2024Article
- Omics Studies of Specialized Cells and Stem Cells under Microgravity Conditions.International journal of molecular sciences · 2024Review
- Simulated lunar microgravity transiently arrests growth and induces osteocyte-chondrocyte lineage differentiation in human Wharton's jelly stem cells.NPJ microgravity · 2024Article
- Conserved mechanisms of self-renewal and pluripotency in mouse and human ESCs regulated by simulated microgravity using a 3D clinostat.Cell death discovery · 2024Article
- Hypergravity enhances RBM4 expression in human bone marrow-derived mesenchymal stem cells and accelerates their differentiation into neurons.Regenerative therapy · 2023Article
- EuniceScope: Low-Cost Imaging Platform for Studying Microgravity Cell Biology.IEEE open journal of engineering in medicine and biology · 2023Article
- Multiscale modeling in the framework of biological systems and its potential for spaceflight biology studies.iScience · 2022Review
- Enhanced self-renewal of human pluripotent stem cells by simulated microgravity.NPJ microgravity · 2022Article
- Differential Single Cell Responses of Embryonic Stem Cells Versus Embryoid Bodies to Gravity Mechanostimulation.Stem cells and development · 2022Article
- RCCS Bioreactor-Based Modeled Microgravity Affects Gastric Cancer Cells and Improves the Chemotherapeutic Effect.Membranes · 2022Article
- Editorial: The Regulating Mechanisms of Development, Growth, and Metabolism: From Ground to Space.Frontiers in cell and developmental biology · 2022Article
- Effects of Simulated Microgravity on Wild Type and Marfan hiPSCs-Derived Embryoid Bodies.Cellular and molecular bioengineering · 2021Article
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 1 country.
Funding
Abstract
objectiveDespite a great number of studies analysing the effects of microgravity on stem cell proliferation and differentiation, few of them have focused on real-time imaging estimates in space. Herein, we utilized the TZ-1 cargo spacecraft, automatic cell culture equipment and live cell imaging techniques to examine the effects of real microgravity on the proliferation and differentiation of mouse embryonic stem cells (mESCs). MATERIALS AND
methodsOct4-GFP, Brachyury-GFP mESC and Oct4-GFP mESC-derived EBs were used as experimental samples in the TZ-1 spaceflight mission. These samples were seeded into chambers, cultured in an automatic cell culture device and were transported into space during the TZ-1 mission. Over 15 days of spaceflight, bright field and fluorescent images of cell growth were taken in micrography, and the medium was changed every day. Real-time image data were transferred to the ground for analysis.
resultsSpace microgravity maintains stemness and long-term survival of mESCs, promising 3D aggregate formation. Although microgravity did not significantly prevent the migration of EBs on the ECM substrate, it did prevent terminal differentiation of cells.
conclusionsThis study demonstrates that space microgravity might play a potential role in supporting 3D cell growth and maintenance of stemness in embryonic stem cells, while it may negatively affect terminal differentiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.