ArticleChemical science2016
Oriented assembly of invisible probes: towards single mRNA imaging in living cells.
Article in Chemical science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- A reversible plasmonic nanoprobe for dynamic imaging of intracellular pH during endocytosis.Chemical science · 2022Article
- Ratiometric fluorescent 3D DNA walker and catalyzed hairpin assembly for determination of microRNA.Mikrochimica acta · 2020Article
- mRNA Detection with Fluorescence-base Imaging Techniques for Arthritis Diagnosis.Journal of rheumatology research · 2019Article
- A single promoter system co-expressing RNA sensor with fluorescent proteins for quantitative mRNA imaging in living tumor cells.Chemical science · 2019Article
- A low cost mobile phone dark-field microscope for nanoparticle-based quantitative studies.Biosensors & bioelectronics · 2018Article
- Plasmon-induced light concentration enhanced imaging visibility as observed by a composite-field microscopy imaging system.Chemical science · 2016Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Due to the complexity of biological systems and the ultralow concentration of analytes, improving the signal-to-noise ratio and lowering the limit of detection to allow highly sensitive detection is key to biomolecule analysis, especially intracellular analysis. Here, we present a method for highly sensitive imaging of mRNA in living cells by using novel invisible oriented probes to construct a turn-on signal generation mechanism from zero background. Two DNA probes (S1 and S2) are asymmetrically modified on two small gold nanoparticles (AuNPs) with a diameter of 20 nm. The hybridization of the two DNA probes with a single target mRNA leads to the formation of an AuNP dimer which shows a prominent plasmonic coupling effect. It generates a strong scattering signal from zero-background under a dark-field spectral analysis system. The unique design of the oriented assembly dimer has the ability to easily discriminate the target signal from the inherent cellular background noise in intracellular detection, thus making this approach a valuable technique for imaging single survivin mRNA and monitoring the distribution of survivin mRNA in tumor cells.
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Registered trials
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