Evidence map›Paper›PMID 29997128›Full record

ArticleJournal of medical genetics2019

Exosomes derived from exhausted CD8+ T cells impaired the anticancer function of normal CD8+ T cells.

Xiaochen Wang, Haiyuan Shen, Qifeng He, Wenfang Tian, Anliang Xia, Xiao-Jie Lu

RetractedAbstract readRetracted Publication
PubMed Publisher
In one paragraph

Article in Journal of medical genetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 60 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
60citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

60 citing papers in PubMed, 1 synthesis or guideline pooled it.

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  12. The role of regulatory T cells in vitiligo and therapeutic advances: a mini-review.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2024
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  13. Extracellular vesicles as tools and targets in therapy for diseases.Signal transduction and targeted therapy · 2024
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors.

Xiaochen Wang *Liver Transplantation Center, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.ORCID 0000-0001-8917-0695
Haiyuan Shen *Liver Transplantation Center, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Qifeng He *Liver Transplantation Center, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Wenfang TianDepartment of Hepatobiliary Surgery, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, China.
Anliang XiaLiver Transplantation Center, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Xiao-Jie LuLiver Transplantation Center, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundPrevious studies suggested that diverse cells in cancer microenvironment can interact with CD8+ T cells via exosomes. We designed this study to explore the potential interaction between exhausted CD8+ T cells and normal CD8+ T cells via exosome.

methodsFluorescence activated cell sorting was used to get PD1+TIM3+/PD1-TIM3-CD8+ T cells. Exosomes from the cell culture medium were collected by ultracentrifugation. Microarrays were performed to analyse the lncRNA expression profile in exosomes.

resultsFunctional exhausted CD8+ T cells could secrete vast exosomes, which can be uptake by normal CD8+ T cells, and impaired their proliferation (Ki67), cell activity (CD69) and the production of cytokines such as interferon-γ and interleukin-2. Microarray detection identified 257 candidate lncRNAs differently expressed in exosomes derived from exhausted CD8+ T cells and non-exhausted CD8+ T cells. Functional enrichment analysis indicated that these lncRNAs actively participated in the regulation of diverse process of CD8+ T cell activity, like metabolism, gene expression, biosynthetic process and so forth.

conclusionsThe exosomes derived from exhausted CD8+ T cells could be uptake by non-exhausted CD8+ T cells and subsequently impaired the function of receipt cells. Exosomes secreted from exhausted CD8+ T cells have distinct lncRNA expression profiles which are significantly different from those in exosomes secreted by non-exhausted CD8+ T cells.

Indexed as

BiomarkersCD8-Positive T-LymphocytesExosomesGene Expression ProfilingGene Expression Regulation, NeoplasticHumansImmunophenotypingLymphocytes, Tumor-InfiltratingMicroRNAsNeoplasmsRNA, Long NoncodingRNA, MessengerTumor MicroenvironmentBiomarkersMicroRNAsRNA, Long NoncodingRNA, MessengerCD8+ T CellsexosomelncRNAT cell exhaustion

Identifiers

PMID29997128

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.