Evidence map›Paper›PMID 29996913›Full record

ArticleBMC research notes2018

Development of a laboratorial platform for diagnosis of schistosomiasis mansoni by PCR-ELISA.

Carolina Senra, Luciana Inácia Gomes, Liliane Maria Vidal Siqueira, Paulo Marcos Zech Coelho, Ana Rabello, Edward Oliveira

Open access · goldAbstract read
In one paragraph

Article in BMC research notes, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed, 1 pooled it
1.2field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 1 synthesis or guideline pooled it, 11 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Article
  4. Article
  5. Article
  6. Review
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Carolina SenraClinical Research and Public Politics in Infectious and Parasitic Diseases, Instituto René Rachou, Oswaldo Cruz Foundation (IRR/FIOCRUZ), Av. Augusto de Lima, 1715, Belo Horizonte, MG, 30190-002, Brazil.
Luciana Inácia GomesClinical Research and Public Politics in Infectious and Parasitic Diseases, Instituto René Rachou, Oswaldo Cruz Foundation (IRR/FIOCRUZ), Av. Augusto de Lima, 1715, Belo Horizonte, MG, 30190-002, Brazil.
Liliane Maria Vidal SiqueiraBiology of the Schistosoma mansoni and its Interaction with Host, Instituto René Rachou, Oswaldo Cruz Foundation (IRR/FIOCRUZ), Av. Augusto de Lima, 1715, Belo Horizonte, MG, 30190-002, Brazil.
Paulo Marcos Zech CoelhoBiology of the Schistosoma mansoni and its Interaction with Host, Instituto René Rachou, Oswaldo Cruz Foundation (IRR/FIOCRUZ), Av. Augusto de Lima, 1715, Belo Horizonte, MG, 30190-002, Brazil.
Ana RabelloClinical Research and Public Politics in Infectious and Parasitic Diseases, Instituto René Rachou, Oswaldo Cruz Foundation (IRR/FIOCRUZ), Av. Augusto de Lima, 1715, Belo Horizonte, MG, 30190-002, Brazil.
Edward OliveiraClinical Research and Public Politics in Infectious and Parasitic Diseases, Instituto René Rachou, Oswaldo Cruz Foundation (IRR/FIOCRUZ), Av. Augusto de Lima, 1715, Belo Horizonte, MG, 30190-002, Brazil. edwardjo@minas.fiocruz.br.ORCID http://orcid.org/0000-0002-0042-5178
Fundação Oswaldo Cruz · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveWe developed a laboratorial platform to release a commercial platform used in the PCR-ELISA for the molecular diagnosis of schistosomiasis mansoni. On following, PCR-ELISA platform laboratorial was evaluated in 206 feces samples collected of individual living in a Brazilian low endemicity area.

resultsThe PCR-ELISA laboratorial platform indicated a prevalence rate of 25.2%, which was higher than the Kato-Katz technique (18.4%) and lower than the commercial platform (30.1%). Considering Kato-Katz technique as the reference, there were 97.4% and 91.1% of relative sensitivity and specificity rates, respectively. The laboratorial platform presented good precision, performance diagnostic, and can be used in replacement to the commercial platform for diagnosis of schistosomiasis by PCR-ELISA.

Indexed as

Enzyme-Linked Immunosorbent AssayPolymerase Chain ReactionAdolescentAdultAgedAged, 80 and overAnimalsBrazilFecesFemaleHumansMaleMiceMiddle AgedParasite Egg CountPrevalenceDiagnosisDisease controlPCR-ELISASchistosoma mansoniSchistosomiasis

Identifiers

PMID29996913
PMCPMC6042422
OpenAlexW2875148821

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.