Evidence map›Paper›PMID 29996898›Full record

ArticleBMC research notes2018

Effects of lyophilization and storage temperature on Wuchereria bancrofti antigen sensitivity and stability.

Edem Y Agbozo, Edward Dumashie, Daniel A Boakye, Dziedzom K de Souza

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC research notes, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Edem Y AgbozoAccra Technical University, Accra, Ghana.
Edward DumashieDepartment of Parasitology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, Ghana.
Daniel A BoakyeDepartment of Parasitology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, Ghana.
Dziedzom K de SouzaDepartment of Parasitology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, Ghana. ddesouza@noguchi.ug.edu.gh.

Funding

European and Developing Countries Clinical Trials Partnership 98595
6 · The paper itself

Abstract

objectiveAntigen-based rapid diagnostic tests for Lymphatic filariasis (LF) do not come with external quality control (QC) materials, and research and disease control programmes rely on stored positive samples. This study was undertaken to evaluate the use of lyophilized Wuchereria bancrofti antigen positive plasma samples to serve as QC materials for LF diagnostic tests. 10 well characterized W. bancrofti positive samples were lyophilized and stored at 4, 28 and 40 °C. The samples were evaluated using the Alere Filariasis Test Strips before lyophilization, and after 1 and 3 months of storage. The sensitivity and stability of the lyophilized samples were evaluated.

resultsThe results revealed a loss of sensitivity and stability with increasing temperature and duration of storage. The results are further discussed in terms of the use of dried blood spot (DBS) in diagnostic studies on LF, and the need for thoughtful DBS preparation and storage.

Indexed as

Freeze DryingAnimalsAntigens, HelminthElephantiasis, FilarialTemperatureWuchereria bancroftiAntigens, HelminthDried blood spotsFreeze-dryingLyophilizationWuchereria bancrofti antigen

Identifiers

PMID29996898
PMCPMC6042357

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.