ArticleMolecular microbiology2018
Aspartate deficiency limits peptidoglycan synthesis and sensitizes cells to antibiotics targeting cell wall synthesis in Bacillus subtilis.
Article in Molecular microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 41 citations in OpenAlex.
- Regulation of PBP2x Surface Localization byPathogens (Basel, Switzerland) · 2026Article
- Emerging therapeutic drug targets in amino acid and protein biosynthesis pathways against ESKAPE and non-ESKAPE pathogens: a comprehensive review.Archives of microbiology · 2026Review
- Molecular interplay between peptidoglycan integrity and outer membrane asymmetry in maintaining cell envelope homeostasis.Journal of bacteriology · 2025Article
- Defects in anaplerotic metabolism sensitizeMicrobiology spectrum · 2025Article
- Chemical Composition of Toona sinensis Extract and its Antibacterial, Antibiofilm, and Antioxidant Activities, with Antibacterial Mechanisms against Listeria monocytogenes ATCC 19112.Plant foods for human nutrition (Dordrecht, Netherlands) · 2025Article
- Regulation of antibiotic persistence and pathogenesis in Acinetobacter baumannii by glutamate and histidine metabolic pathways.BMC microbiology · 2025Article
- Article
- Metabolic rewiring enables ammonium assimilation via a non-canonical fumarate-based pathway.Microbial biotechnology · 2024Article
- Experimentally evolvedMicrobiology (Reading, England) · 2024Article
- Resource partitioning and amino acid assimilation in a terrestrial geothermal spring.The ISME journal · 2023Article
- TerC proteins function during protein secretion to metalate exoenzymes.Nature communications · 2023Article
- TerC Proteins Function During Protein Secretion to Metalate Exoenzymes.Research square · 2023Article
- TerC Proteins Function During Protein Secretion to Metalate Exoenzymes.bioRxiv : the preprint server for biology · 2023Article
- Review
- Bacterial metabolism and susceptibility to cell wall-active antibiotics.Advances in microbial physiology · 2023Review
- The VarA-CsrA regulatory pathway influences cell shape in Vibrio cholerae.PLoS genetics · 2022Article
- Hydroxyl Radical Overproduction in the Envelope: an Achilles' Heel in Peptidoglycan Synthesis.Microbiology spectrum · 2022Article
- NMR Hydrophilic Metabolomic Analysis of Bacterial Resistance Pathways Using Multivalent Antimicrobials with Challenged and Unchallenged Wild Type and Mutated Gram-Positive Bacteria.International journal of molecular sciences · 2021Article
- Elucidating the Antimycobacterial Mechanism of Action of Ciprofloxacin Using Metabolomics.Microorganisms · 2021Article
- Resource sharing between central metabolism and cell envelope synthesis.Current opinion in microbiology · 2021Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Peptidoglycan synthesis is an important target for antibiotics and relies on intermediates derived from central metabolism. As a result, alterations of metabolism may affect antibiotic sensitivity. An aspB mutant is auxotrophic for aspartate (Asp) and asparagine (Asn) and lyses when grown in Difco sporulation medium (DSM), but not in LB medium. Genetic and physiological studies, supported by amino acid analysis, reveal that cell lysis in DSM results from Asp limitation due to a relatively low Asp and high glutamate (Glu) concentrations, with Glu functioning as a competitive inhibitor of Asp uptake by the major Glu/Asp transporter GltT. Lysis can be specifically suppressed by supplementation with 2,6-diaminopimelate (DAP), which is imported by two different cystine uptake systems. These studies suggest that aspartate limitation depletes the peptidoglycan precursor meso-2,6-diaminopimelate (mDAP), inhibits peptidoglycan synthesis, upregulates the cell envelope stress response mediated by σ
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.