Evidence map›Paper›PMID 29992724›Full record

ArticleAngewandte Chemie (International ed. in English)2018

Surface Immobilization of Redox-Labile Fluorescent Probes: Enabling Single-Cell Co-Profiling of Aerobic Glycolysis and Oncogenic Protein Signaling Activities.

Zhonghan Li, Hanjun Cheng, Shiqun Shao, Xiang Lu, Li Mo, Jonathan Tsang, Pu Zeng, Zhili Guo, Siwen Wang, David A Nathanson and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Angewandte Chemie (International ed. in English), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Non-Mass Spectrometric Targeted Single-Cell Metabolomics.Trends in analytical chemistry : TRAC · 2023
    Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Zhonghan LiDepartment of Chemistry, University of California, Riverside, CA, 92521, USA.
Hanjun ChengDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA, 90095, USA.
Shiqun ShaoDepartment of Chemistry, University of California, Riverside, CA, 92521, USA.
Xiang LuDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA, 90095, USA.
Li MoDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA, 90095, USA.
Jonathan TsangDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA, 90095, USA.
Pu ZengDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA, 90095, USA.
Zhili GuoDepartment of Chemistry, University of California, Riverside, CA, 92521, USA.
Siwen WangDepartment of Chemistry, University of California, Riverside, CA, 92521, USA.
David A NathansonDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA, 90095, USA.
James R HeathInstitute for Systems Biology, Seattle, WA, 98109, USA.
Wei WeiDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA, 90095, USA.
Min XueDepartment of Chemistry, University of California, Riverside, CA, 92521, USA.ORCID 0000-0002-8136-6551

Funding

UCLA SPORE in Brain CancerP50CA211015 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI David A. Nathanson · 2017 to 2026
$25.2M
Repurposing of cancer therapeutics for treatment of patients with COVID-19 diseaseU54CA199090 · NCI · INSTITUTE FOR SYSTEMS BIOLOGY · PI HEATH, JAMES R., RIBAS, ANTONI · 2015 to 2020
$11.4M
Steady states and cellular transitions associated with carcinogenesis and tumorprogressionU01CA217655 · NCI · INSTITUTE FOR SYSTEMS BIOLOGY · PI HEATH, JAMES R., WEI, WEI · 2017 to 2021
$3.9M
NCI NIH HHS P50 CA211015NCI NIH HHS U01 CA217655NCI NIH HHS U54 CA199090
6 · The paper itself

Abstract

An analytical method is described for profiling lactate production in single cells via the use of coupled enzyme reactions on surface-grafted resazurin molecules. The immobilization of the redox-labile probes was achieved through chemical modifications on resazurin, followed by bio-orthogonal click reactions. The lactate detection was demonstrated to be sensitive and specific. The method was incorporated into a single-cell barcode chip for simultaneous quantification of aerobic glycolysis activities and oncogenic signaling phosphoproteins in cancer. The interplay between glycolysis and oncogenic signaling activities was interrogated on a glioblastoma cell line. Results revealed a drug-induced oncogenic signaling reliance accompanying shifted metabolic paradigms. A drug combination that exploits this induced reliance exhibited synergistic effects in growth inhibition.

Indexed as

GlycolysisSignal TransductionBiosensing TechniquesCell Line, TumorClick ChemistryFluorescent DyesHumansLactic AcidModels, MolecularNeoplasmsOncogene ProteinsOxidation-ReductionSingle-Cell AnalysisSpectrometry, FluorescenceFluorescent DyesLactic AcidOncogene Proteinsaerobic glycolysiscancerfluorescent probesmetabolismsingle cell analysis

Identifiers

PMID29992724
PMCPMC6443403

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.