ArticleScientific reports2018
Extracellular DNA release from the genome-reduced pathogen Mycoplasma hyopneumoniae is essential for biofilm formation on abiotic surfaces.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 27 citations in OpenAlex.
- Rethinking Mycoplasma hyopneumoniae adhesion: a glycocalyx-mediated infectious niche model in porcine respiratory infection.Veterinary research · 2026Review
- Imaging nucleic acids in biofilms.Essays in biochemistry · 2026Review
- Comparative evaluation and validation of rapid quantification methods for Mycoplasma hyopneumoniae: development of a PMA-based viability qPCR assay.Scientific reports · 2026Article
- Review
- Biofilm characterisation of Mycoplasma bovis co-cultured with Trueperella pyogenes.Veterinary research · 2025Article
- Innovative Methodology for Antimicrobial Susceptibility Determination inMicroorganisms · 2024Article
- Bacterial aggregation triggered by low-level antibiotic-mediated lysis.NPJ biofilms and microbiomes · 2024Article
- Differential domains and endoproteolytic processing in dominant surface proteins of unknown function fromHeliyon · 2023Article
- The Role of Biofilms in the Pathogenesis of Animal Bacterial Infections.Microorganisms · 2023Review
- Review
- Novel mycoplasma nucleomodulin MbovP475 decreased cell viability by regulating expression of CRYAB and MCF2L2.Virulence · 2022Article
- Genotyping and biofilm formation of Mycoplasma hyopneumoniae and their association with virulence.Veterinary research · 2022Article
- NADH oxidase of Mycoplasma hyopneumoniae functions as a potential mediator of virulence.BMC veterinary research · 2022Article
- Mycoplasmas as Host Pantropic and Specific Pathogens: Clinical Implications, Gene Transfer, Virulence Factors, and Future Perspectives.Frontiers in cellular and infection microbiology · 2022Review
- TheFrontiers in microbiology · 2022Review
- Review
- Attenuation ofFrontiers in veterinary science · 2021Article
- Mycoplasma bovisJournal of bacteriology · 2020Article
- Article
- Pathogenicity & virulence ofVirulence · 2020Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mycoplasma hyopneumoniae is an economically devastating, globally disseminated pathogen that can maintain a chronic infectious state within its host, swine. Here, we depict the events underpinning M. hyopneumoniae biofilm formation on an abiotic surface and demonstrate for the first time, biofilms forming on porcine epithelial cell monolayers and in the lungs of pigs, experimentally infected with M. hyopneumoniae. Nuclease treatment prevents biofilms forming on glass but not on porcine epithelial cells indicating that extracellular DNA (eDNA), which localises at the base of biofilms, is critical in the formation of these structures on abiotic surfaces. Subpopulations of M. hyopneumoniae cells, denoted by their ability to take up the dye TOTO-1 and release eDNA, were identified. A visually distinct sub-population of pleomorphic cells, that we refer to here as large cell variants (LCVs), rapidly transition from phase dark to translucent "ghost" cells. The translucent cells accumulate the membrane-impermeable dye TOTO-1, forming readily discernible membrane breaches immediately prior to lysis and the possible release of eDNA and other intracellular content (public goods) into the extracellular environment. Our novel observations expand knowledge of the lifestyles adopted by this wall-less, genome-reduced pathogen and provide further insights to its survival within farm environments and swine.
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