ArticleScientific reports2018
Volumetric, Nanoscale Optical Imaging of Mouse and Human Kidney via Expansion Microscopy.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 45 citations in OpenAlex.
- Article
- Super-resolved optical imaging, reconstruction, and spatial analysis of whole mouse glomeruli via the Glomerulus Mapping and Analysis Pipeline.Kidney international · 2025Article
- Clinical translation potential of self-inspired live-cell super-resolution microscopy.Clinical and translational medicine · 2025Article
- Ultrastructure expansion microscopy (U-ExM) of mouse and human kidneys for analysis of subcellular structures.Cytoskeleton (Hoboken, N.J.) · 2024Article
- On-demand expansion fluorescence and photoacoustic microscopy (ExFLPAM).Photoacoustics · 2024Article
- Development of an automated estimation of foot process width using deep learning in kidney biopsies from patients with Fabry, minimal change, and diabetic kidney diseases.Kidney international · 2024Article
- Magnify is a universal molecular anchoring strategy for expansion microscopy.Nature biotechnology · 2023Article
- Beyond conventional microscopy: Observing kidney tissues by means of fourier ptychography.Frontiers in physiology · 2023Article
- Three-dimensional visualization of the cardiac ryanodine receptor clusters and the molecular-scale fraying of dyads.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Article
- Fluorescent labeling of abundant reactive entities (FLARE) for cleared-tissue and super-resolution microscopy.Nature protocols · 2022Review
- Quantitative Analyses of Foot Processes, Mitochondria, and Basement Membranes by Structured Illumination Microscopy Using Elastica-Masson- and Periodic-Acid-Schiff-Stained Kidney Sections.Kidney international reports · 2021Article
- Apical-basal polarity regulators are essential for slit diaphragm assembly and endocytosis in Drosophila nephrocytes.Cellular and molecular life sciences : CMLS · 2021Article
- Advances in fluorescence microscopy techniques to study kidney function.Nature reviews. Nephrology · 2021Review
- Superresolving the kidney-a practical comparison of fluorescence nanoscopy of the glomerular filtration barrier.Analytical and bioanalytical chemistry · 2021Article
- Complexities of the glomerular basement membrane.Nature reviews. Nephrology · 2021Review
- Imaging the kidney: from light to super-resolution microscopy.Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association · 2021Review
- Global transcriptomic changes occur in aged mouse podocytes.Kidney international · 2020Article
- Article
- Dual lineage tracing shows that glomerular parietal epithelial cells can transdifferentiate toward the adult podocyte fate.Kidney international · 2019Article
- Multi-immersion open-top light-sheet microscope for high-throughput imaging of cleared tissues.Nature communications · 2019Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Although light microscopy is a powerful tool for the assessment of kidney physiology and pathology, it has traditionally been unable to resolve structures separated by less than the ~250 nm diffraction limit of visible light. Here, we report on the optimization, validation, and application of a recently developed super-resolution fluorescence microscopy method, called expansion microscopy (ExM), for volumetric interrogation of mouse and human kidney tissue with 70-75 nm lateral and ~250 nm axial spatial resolution. Using ExM with a standard confocal microscope, we resolve fine details of structures that have traditionally required visualization by electron microscopy, including podocyte foot processes, the glomerular basement membrane, and the cytoskeleton. This inexpensive and accessible approach to volumetric, nanoscale imaging enables visualization of fine structural details of kidney tissues that were previously difficult or impossible to measure by conventional methodologies.
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Registered trials
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