ArticleScientific reports2018
Quantitative fibre analysis of single-molecule localization microscopy data.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
- Photonic waveguide chip-based nanoscopy visualizes rearrangements of the cortical actin cytoskeleton in activated Jurkat T cells.Science advances · 2026Article
- Quantitative Single-Molecule Localization Microscopy.Annual review of biophysics · 2023Review
- Peptide-PAINT Using a Transfected-Docker Enables Live- and Fixed-Cell Super-Resolution Imaging.Small methods · 2023Article
- The anatomy of transcriptionally active chromatin loops in Drosophila primary spermatocytes using super-resolution microscopy.PLoS genetics · 2023Article
- Cluster analysis for localisation-based data sets: dos and don'ts when quantifying protein aggregates.Frontiers in bioinformatics · 2023Review
- resPAINT: Accelerating Volumetric Super-Resolution Localisation Microscopy by Active Control of Probe Emission.Angewandte Chemie (International ed. in English) · 2022Article
- resPAINT: Accelerating Volumetric Super-Resolution Localisation Microscopy by Active Control of Probe Emission.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2022Article
- Simple methods for quantifying super-resolved cortical actin.Scientific reports · 2022Article
- Protocol for multicolor three-dimensional dSTORM data analysis using MATLAB-based script package Grafeo.STAR protocols · 2021Article
- Computational analyses reveal spatial relationships between nuclear pore complexes and specific lamins.The Journal of cell biology · 2021Article
- Density Distribution Maps: A Novel Tool for Subcellular Distribution Analysis and Quantitative Biomedical Imaging.Sensors (Basel, Switzerland) · 2021Article
- Single-molecule localization microscopy.Nature reviews. Methods primers · 2021Article
- Super-Resolution Imaging Approaches for Quantifying F-Actin in Immune Cells.Frontiers in cell and developmental biology · 2021Review
- Adaptive multiorientation resolution analysis of complex filamentous network images.Bioinformatics (Oxford, England) · 2020Article
- A Review of Super-Resolution Single-Molecule Localization Microscopy Cluster Analysis and Quantification Methods.Patterns (New York, N.Y.) · 2020Review
- Ultraprecise single-molecule localization microscopy enables in situ distance measurements in intact cells.Science advances · 2020Article
- Review
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Single molecule localization microscopy (SMLM) methods produce data in the form of a spatial point pattern (SPP) of all localized emitters. Whilst numerous tools exist to quantify molecular clustering in SPP data, the analysis of fibrous structures has remained understudied. Taking the SMLM localization coordinates as input, we present an algorithm capable of tracing fibrous structures in data generated by SMLM. Based upon a density parameter tracing routine, the algorithm outputs several fibre descriptors, such as number of fibres, length of fibres, area of enclosed regions and locations and angles of fibre branch points. The method is validated in a variety of simulated conditions and experimental data acquired using the image reconstruction by integrating exchangeable single-molecule localization (IRIS) technique. For this, the nanoscale architecture of F-actin at the T cell immunological synapse in both untreated and pharmacologically treated cells, designed to perturb actin structure, was analysed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.