Evidence map›Paper›PMID 29988552›Full record

ArticleFEBS open bio2018

Effect of sequential C-terminal tryptophans on green fluorescent protein fluorescence.

Shirou Tsuchida, Takumi Kanashiki, Shuhei Izumiya, Takuya Ichikawa, Ryusuke Kurosawa, Naoya Hamaue, Takashi Aoki

Open access · goldAbstract read
In one paragraph

Article in FEBS open bio, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 96% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Shirou TsuchidaDepartment of Molecular Biosciences School of Pharmaceutical Sciences Health Sciences University of Hokkaido Ishikari-Tobetsu Japan.
Takumi KanashikiDepartment of Molecular Biosciences School of Pharmaceutical Sciences Health Sciences University of Hokkaido Ishikari-Tobetsu Japan.
Shuhei IzumiyaDepartment of Molecular Biosciences School of Pharmaceutical Sciences Health Sciences University of Hokkaido Ishikari-Tobetsu Japan.
Takuya IchikawaDepartment of Molecular Biosciences School of Pharmaceutical Sciences Health Sciences University of Hokkaido Ishikari-Tobetsu Japan.
Ryusuke KurosawaDepartment of Molecular Biosciences School of Pharmaceutical Sciences Health Sciences University of Hokkaido Ishikari-Tobetsu Japan.
Naoya HamaueDepartment of Molecular Biosciences School of Pharmaceutical Sciences Health Sciences University of Hokkaido Ishikari-Tobetsu Japan.
Takashi AokiDepartment of Molecular Biosciences School of Pharmaceutical Sciences Health Sciences University of Hokkaido Ishikari-Tobetsu Japan.
Health Sciences University of Hokkaido · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The effect of the addition of sequential C-terminal tryptophan residues on the fluorescence intensity of GFP was investigated. Tandem repeats of six tryptophan residues markedly decreased fluorescence intensity. This phenomenon is likely to occur because of the inhibition of GFP folding, resulting in insolubility. Exploiting this phenomenon, we constructed a cloning vector that facilitates the identification of recombinant colonies of

Indexed as

cloning vectorC terminusEscherichia coligreen fluorescent proteintryptophan

Identifiers

PMID29988552
PMCPMC6026694
OpenAlexW2801910720

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.