ArticleFrontiers in microbiology2018
Evaluating Established Methods for Rumen 16S rRNA Amplicon Sequencing With Mock Microbial Populations.
Article in Frontiers in microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 48 citations in OpenAlex.
- Manually weighted taxonomy classifiers improve species-specific rumen microbiome analysis compared to unweighted or average weighted taxonomy classifiers.Scientific reports · 2025Article
- Effect of Dietary Addition ofAnimals : an open access journal from MDPI · 2025Article
- Microbial Populations in Ruminal Liquid Samples from Young Beefmaster Bulls at Both Extremes of RFI Values.Microorganisms · 2023Article
- Insufficiency in functional genomics studies, data, and applications: A case study of bio-prospecting research in ruminant microbiome.Frontiers in genetics · 2022Review
- Application of MinION Amplicon Sequencing to Buccal Swab Samples for Improving Resolution and Throughput of Rumen Microbiota Analysis.Frontiers in microbiology · 2022Article
- Differences in the Composition of the Rumen Microbiota of Finishing Beef Cattle Divergently Ranked for Residual Methane Emissions.Frontiers in microbiology · 2022Article
- The potential for mitigation of methane emissions in ruminants through the application of metagenomics, metabolomics, and other -OMICS technologies.Journal of animal science · 2021Review
- Refinement of microbiota analysis of specimens from patients with respiratory infections using next-generation sequencing.Scientific reports · 2021Article
- Elucidating biofilm diversity on water lily leaves through 16S rRNA amplicon analysis: Comparison of four DNA extraction kits.Applications in plant sciences · 2021Article
- Amplicon-sequencing of raw milk microbiota: impact of DNA extraction and library-PCR.Applied microbiology and biotechnology · 2021Article
- Ruminal Degradation of Rumen-Protected Glucose Influences the Ruminal Microbiota and Metabolites in Early-Lactation Dairy Cows.Applied and environmental microbiology · 2021Article
- Evaluation of Established Methods for DNA Extraction and Primer Pairs Targeting 16S rRNA Gene for Bacterial Microbiota Profiling of Olive Xylem Sap.Frontiers in plant science · 2021Article
- Presence of Adult Companion Goats Favors the Rumen Microbial and Functional Development in Artificially Reared Kids.Frontiers in veterinary science · 2021Article
- Efficient and stable metabarcoding sequencing data using a DNBSEQ-G400 sequencer validated by comprehensive community analyses.GigaByte (Hong Kong, China) · 2021Article
- Sward type alters the relative abundance of members of the rumen microbial ecosystem in dairy cows.Scientific reports · 2020Article
- Article
- Synthetic Sequencing Standards: A Guide to Database Choice for Rumen Microbiota Amplicon Sequencing Analysis.Frontiers in microbiology · 2020Article
- Inhibitory Effect of a Microecological Preparation on Azoxymethane/Dextran Sodium Sulfate-Induced Inflammatory Colorectal Cancer in Mice.Frontiers in oncology · 2020Article
- Freshwater Sediment Microbial Communities Are Not Resilient to Disturbance From Agricultural Land Runoff.Frontiers in microbiology · 2020Article
- Replacing Barley and Soybean Meal With By-products, in a Pasture Based Diet, Alters Daily Methane Output and the Rumen Microbial CommunityFrontiers in microbiology · 2020Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The rumen microbiome scientific community has utilized amplicon sequencing as an aid in identifying potential community compositional trends that could be used as an estimation of various production and performance traits including methane emission, animal protein production efficiency, and ruminant health status. In order to translate rumen microbiome studies into executable application, there is a need for experimental and analytical concordance within the community. The objective of this study was to assess these factors in relation to selected currently established methods for 16S phylogenetic community analysis on a microbial community standard (MC) and a DNA standard (DS; ZymoBIOMICS
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.