ArticleNature protocols2018
In vitro generation of mouse polarized embryo-like structures from embryonic and trophoblast stem cells.
Article in Nature protocols, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- ROCK inhibition suppresses host blastomere-mediated elimination and enhances trophoblast stem cell contribution in mouse chimeras.Stem cell reports · 2026Article
- Replication stress primes a trophectoderm fate in embryonic stem cells.Cell death discovery · 2026Article
- Stem Cell-Derived Embryo Models: Potential Platforms for Investigating Early Development and Embryotoxicity.International journal of stem cells · 2025Review
- Lentivirus-Mediated Trophoblast-Specific Deptor Knockdown Increases Transplacental System A and System L Amino Acid Transport and Fetal Growth in Mice.Function (Oxford, England) · 2025Article
- Insulin Resistance Is a Modifying Factor for Parkinson's Disease.Movement disorders : official journal of the Movement Disorder Society · 2025Article
- Assembly of complete mouse embryo models from embryonic and induced stem cell types in vitro.Nature protocols · 2023Review
- A chemo-mechanical model of endoderm movements driving elongation of the amniote hindgut.bioRxiv : the preprint server for biology · 2023Article
- Efficient generation of ETX embryoids that recapitulate the entire window of murine egg cylinder development.Science advances · 2023Article
- Periodic formation of epithelial somites from human pluripotent stem cells.Nature communications · 2022Article
- Embryotoxic effects of tribromophenol on early post-implantation development of mouse embryos in vitro.Environmental science and pollution research international · 2022Article
- Assessing the Wnt-reactivity of cytonemes of mouse embryonic stem cells using a bioengineering approach.STAR protocols · 2021Article
- Review
- Machine learning-assisted high-content analysis of pluripotent stem cell-derived embryos in vitro.Stem cell reports · 2021Article
- From embryos to embryoids: How external signals and self-organization drive embryonic development.Stem cell reports · 2021Review
- Pluripotency state regulates cytoneme selectivity and self-organization of embryonic stem cells.The Journal of cell biology · 2021Article
- From Snapshots to Development: Identifying the Gaps in the Development of Stem Cell-based Embryo Models along the Embryonic Timeline.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- Mouse gastrulation: Coordination of tissue patterning, specification and diversification of cell fate.Mechanisms of development · 2020Review
- A novel "embryo-endometrial" adhesion model can potentially predict "receptive" or "non-receptive" endometrium.Journal of assisted reproduction and genetics · 2020Article
- Opening the black box: Stem cell-based modeling of human post-implantation development.The Journal of cell biology · 2019Review
- Insulin resistance compromises midbrain organoid neuronal activity and metabolic efficiency predisposing to Parkinson's disease pathology.Journal of tissue engineeringArticle
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mammalian embryogenesis requires the coordination of embryonic and extra-embryonic tissues to enable implantation into the uterus and post-implantation development to establish the body plan. Mouse embryonic stem cells (ESCs) are a useful tool for studying pluripotent embryonic tissue in vitro. However, they cannot undertake correct embryogenesis alone. Many attempts to model the early embryo in vitro involve the aggregation of ESCs into spheroids of variable size and cell number that undertake germ-layer specification but fail to recapitulate the characteristic architecture and arrangement of tissues of the early embryo. Here, we describe a protocol to generate the first embryo-like structures by directing the assembly of mouse ESCs and extra-embryonic trophoblast stem cells (TSCs) in a 3D extracellular matrix (ECM) into structures we call 'polarized embryo-like structures'. By establishing the medium and culture conditions needed to support the growth of both stem cell types simultaneously, embryonic architecture is generated within 4 d of co-culture. This protocol can be performed by those proficient in standard ESC culture techniques and can be used in developmental studies to investigate the interactions between embryonic and extra-embryonic tissues during mammalian development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.