Evidence map›Paper›PMID 29988095›Full record

ArticleNature methods2018

Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy.

Francesca Pennacchietti, Ekaterina O Serebrovskaya, Aline R Faro, Irina I Shemyakina, Nina G Bozhanova, Alexey A Kotlobay, Nadya G Gurskaya, Andreas Bodén, Jes Dreier, Dmitry M Chudakov and 4 more

Abstract read
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In one paragraph

Article in Nature methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.

0numbers the graph read from it
0cells of the map it votes in
35citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

35 citing papers in PubMed.

  1. Article
  2. A Versatile Tool to Predict and Guide RESOLFT Images Based on Photoswitching, Labelling and Optical Properties.Chemphyschem : a European journal of chemical physics and physical chemistry · 2026
    Article
  3. Review
  4. Article
  5. Article
  6. Article
  7. Crystal structure analysis of oxygen-induced degradation occurring in rsCherry.Acta crystallographica. Section F, Structural biology communications · 2025
    Article
  8. Article
  9. Article
  10. Review
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Review
  17. Photoswitchable Fluorescent Proteins: Mechanisms on Ultrafast Timescales.International journal of molecular sciences · 2022
    Review
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

14 authors.

Francesca PennacchiettiDepartment of Applied Physics and Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden.
Ekaterina O SerebrovskayaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russian Federation.ORCID http://orcid.org/0000-0002-4967-7165
Aline R FaroDepartment of Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY, USA.
Irina I ShemyakinaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russian Federation.
Nina G BozhanovaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russian Federation.ORCID http://orcid.org/0000-0002-2164-5698
Alexey A KotlobayShemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russian Federation.
Nadya G GurskayaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russian Federation.
Andreas BodénDepartment of Applied Physics and Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden.
Jes DreierDepartment of Applied Physics and Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden.ORCID http://orcid.org/0000-0002-9596-5125
Dmitry M ChudakovShemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russian Federation.
Konstantin A LukyanovShemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russian Federation.
Vladislav V VerkhushaDepartment of Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY, USA.
Alexander S MishinShemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russian Federation. mishin@ibch.ru.ORCID http://orcid.org/0000-0002-4935-7030
Ilaria TestaDepartment of Applied Physics and Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden. ilaria.testa@scilifelab.se.ORCID http://orcid.org/0000-0003-4005-4997

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Reversibly photoswitchable fluorescent proteins (rsFPs) are gaining popularity as tags for optical nanoscopy because they make it possible to image with lower light doses. However, green rsFPs need violet-blue light for photoswitching, which is potentially phototoxic and highly scattering. We developed new rsFPs based on FusionRed that are reversibly photoswitchable with green-orange light. The rsFusionReds are bright and exhibit rapid photoswitching, thereby enabling nanoscale imaging of living cells.

Indexed as

Cell LineHumansIntravital MicroscopyKineticsLightLuminescent ProteinsMicroscopy, FluorescenceNanotechnologyPhotochemical ProcessesRecombinant Fusion ProteinsRed Fluorescent ProteinSpectrophotometryLuminescent ProteinsRecombinant Fusion ProteinsRed Fluorescent Protein

Identifiers

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.