Evidence map›Paper›PMID 29988094›Full record

ArticleNature methods2018

Genome-wide SWAp-Tag yeast libraries for proteome exploration.

Uri Weill, Ido Yofe, Ehud Sass, Bram Stynen, Dan Davidi, Janani Natarajan, Reut Ben-Menachem, Zohar Avihou, Omer Goldman, Nofar Harpaz and 15 more

Erratum issuedAbstract read
In one paragraph

Article in Nature methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 110 papers.

0numbers the graph read from it
0cells of the map it votes in
110citing papers in PubMed
8.1field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

110 citing papers in PubMed, 209 citations in OpenAlex.

  1. Role of Pbr1, a putative oxidoreductase, in the ER quality control and folding of yeast Fks1 glucan synthase.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  2. Article
  3. Review
  4. Review
  5. Article
  6. Article
  7. Review
  8. Article
  9. Review
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Review
  17. Article
  18. Article
  19. Article
  20. Article

50 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

25 authors at 6 institutions in 3 countries.

Uri WeillDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
Ido YofeDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
Ehud SassDepartment of Structural Biology, Weizmann Institute of Science, Rehovot, Israel.
Bram StynenDépartement de Biochimie, Faculté de Médecine, Université de Montréal, Montreal, QC, Canada.
Dan DavidiDepartment of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel.
Janani NatarajanInterfaculty Institute of Biochemistry, University of Tübingen, Tübingen, Germany.
Reut Ben-MenachemDepartment of Microbiology and Molecular Genetics, IMRIC, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel.
Zohar AvihouDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
Omer GoldmanDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
Nofar HarpazDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
Silvia ChuartzmanDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
Kiril KniazevDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
Barbara KnoblachDepartment of Cell Biology, University of Alberta, Edmonton, AB, Canada.
Janina LaborenzDepartment of Cell Biology, University of Kaiserslautern, Kaiserslautern, Germany.
Felix BoosDepartment of Cell Biology, University of Kaiserslautern, Kaiserslautern, Germany.
Jacqueline KowarzykDépartement de Biochimie, Faculté de Médecine, Université de Montréal, Montreal, QC, Canada.
Shifra Ben-DorDepartment of Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, Israel.ORCID http://orcid.org/0000-0001-9604-1939
Einat ZalckvarDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
Johannes M HerrmannDepartment of Cell Biology, University of Kaiserslautern, Kaiserslautern, Germany.
Richard A RachubinskiDepartment of Cell Biology, University of Alberta, Edmonton, AB, Canada.ORCID http://orcid.org/0000-0001-9842-4327
Ophry PinesDepartment of Microbiology and Molecular Genetics, IMRIC, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel.
Doron RapaportInterfaculty Institute of Biochemistry, University of Tübingen, Tübingen, Germany.
Stephen W MichnickDépartement de Biochimie, Faculté de Médecine, Université de Montréal, Montreal, QC, Canada.
Emmanuel D LevyDepartment of Structural Biology, Weizmann Institute of Science, Rehovot, Israel.ORCID http://orcid.org/0000-0001-8959-7365
Maya SchuldinerDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel. maya.schuldiner@weizmann.ac.il.ORCID http://orcid.org/0000-0001-9947-115X
Weizmann Institute of Science · ILUniversité de Montréal · CAUniversity of Kaiserslautern · DEHebrew University of Jerusalem · ILUniversity of Alberta · CAUniversity of Tübingen · DE

Funding

European Research Council 646604
6 · The paper itself

Abstract

Yeast libraries revolutionized the systematic study of cell biology. To extensively increase the number of such libraries, we used our previously devised SWAp-Tag (SWAT) approach to construct a genome-wide library of ~5,500 strains carrying the SWAT NOP1promoter-GFP module at the N terminus of proteins. In addition, we created six diverse libraries that restored the native regulation, created an overexpression library with a Cherry tag, or enabled protein complementation assays from two fragments of an enzyme or fluorophore. We developed methods utilizing these SWAT collections to systematically characterize the yeast proteome for protein abundance, localization, topology, and interactions.

Indexed as

Genome, FungalGenomic LibraryGenetic Complementation TestGreen Fluorescent ProteinsMitochondrial ProteinsNuclear ProteinsPromoter Regions, GeneticProtein Interaction MappingProteomeRecombinant Fusion ProteinsRibonucleoproteins, Small NucleolarSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence Tagged SitesGreen Fluorescent ProteinsMitochondrial ProteinsNOP1 protein, S cerevisiaeNuclear ProteinsProteomeRecombinant Fusion ProteinsRibonucleoproteins, Small NucleolarSaccharomyces cerevisiae Proteins

Identifiers

PMID29988094
PMCPMC6076999
OpenAlexW2810266624

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.