ArticleProceedings of the National Academy of Sciences of the United States of America2018
Unrestrained markerless trait stacking in
Article in Proceedings of the National Academy of Sciences of the United States of America, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 93 citations in OpenAlex.
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- Environmental Applications of GM Microorganisms: Tiny Critters Posing Huge Challenges for Risk Assessment and Governance.International journal of molecular sciences · 2025Review
- Progress and challenges in CRISPR/Cas applications in microalgae.Journal of microbiology (Seoul, Korea) · 2025Review
- Leveraging CRISPR Cas9 RNPs and Cre-Frontiers in microbiology · 2025Article
- Review
- EngineeringmLife · 2023Article
- CRISPR-based bioengineering in microalgae for production of industrially important biomolecules.Frontiers in bioengineering and biotechnology · 2023Review
- Genome editing with removable TALEN vectors harboring a yeast centromere and autonomous replication sequence in oleaginous microalga.Scientific reports · 2022Article
- High-throughput selection of cells based on accumulated growth and division using PicoShell particles.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Comprehensive Genome Engineering Toolbox for MicroalgaeACS synthetic biology · 2021Article
- Current perspectives on integrated approaches to enhance lipid accumulation in microalgae.3 Biotech · 2021Review
- Consequences of Mixotrophy on Cell Energetic Metabolism inFrontiers in plant science · 2021Article
- CRISPR/Cas9 Ribonucleoprotein-Based Genome Editing Methodology in the Marine Protozoan ParasiteFrontiers in bioengineering and biotechnology · 2021Article
- Bioengineering of Microalgae: Recent Advances, Perspectives, and Regulatory Challenges for Industrial Application.Frontiers in bioengineering and biotechnology · 2020Review
- Improved lipid productivity inBiotechnology for biofuels · 2020Article
- CRISPR-Cas9 System for Genome Engineering of Photosynthetic Microalgae.Molecular biotechnology · 2019Review
- Genome assembly ofCommunications biology · 2019Article
- CRISPR-Cas ribonucleoprotein mediated homology-directed repair for efficient targeted genome editing in microalgaeBiotechnology for biofuels · 2019Article
- Review
Corrections and comments
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Authors and funding
14 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Robust molecular tool kits in model and industrial microalgae are key to efficient targeted manipulation of endogenous and foreign genes in the nuclear genome for basic research and, as importantly, for the development of algal strains to produce renewable products such as biofuels. While Cas9-mediated gene knockout has been demonstrated in a small number of algal species with varying efficiency, the ability to stack traits or generate knockout mutations in two or more loci are often severely limited by selectable agent availability. This poses a critical hurdle in developing production strains, which require stacking of multiple traits, or in probing functionally redundant gene families. Here, we combine Cas9 genome editing with an inducible Cre recombinase in the industrial alga
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.