ArticleProceedings of the National Academy of Sciences of the United States of America2018
Virion structure and genome delivery mechanism of sacbrood honeybee virus.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 33 citations in OpenAlex.
- Screening and functional analysis of a host membrane protein interacting with the structural protein VP1 of deformed wing virus.Frontiers in microbiology · 2026Article
- Review
- A DNA Plasmid-Based Approach for Efficient Synthesis of Sacbrood Virus Infectious Clones within Host Cells.Viruses · 2023Article
- Large-Scale Application of Double-Stranded RNA Shows Potential for Reduction of Sacbrood Virus Disease inViruses · 2023Article
- Discovery of SNP Molecular Markers and Candidate Genes Associated with Sacbrood Virus Resistance inInternational journal of molecular sciences · 2023Article
- Characterization of Iflavirus in the Red Flour Beetle,Insects · 2023Article
- Molecular Detection and Phylogenetic Analysis of Deformed Wing Virus and Sacbrood Virus Isolated from Pollen.Veterinary sciences · 2023Article
- Nationwide genomic surveillance reveals the prevalence and evolution of honeybee viruses in China.Microbiome · 2023Article
- Interaction between the VP2 protein of deformed wing virus and host snapin protein and its effect on viral replication.Frontiers in microbiology · 2023Article
- Honeybee Iflaviruses Pack Specific tRNA Fragments from Host Cells in Their Virions.Chembiochem : a European journal of chemical biology · 2022Article
- Review
- Development of a Kit for Rapid Immunochromatographic Detection of Sacbrood Virus InfectingViruses · 2021Article
- Lipidomic Profiling Reveals Distinct Differences in Sphingolipids Metabolic Pathway between HealthyInsects · 2021Article
- PROXIMA-1 beamline for macromolecular crystallography measurements at Synchrotron SOLEIL.Journal of synchrotron radiation · 2021Article
- Organization of the Structural Protein Region of La Jolla Virus Isolated from the Invasive Pest InsectViruses · 2021Article
- Capsid opening enables genome release of iflaviruses.Science advances · 2021Article
- Capsid Structure of a Marine Algal Virus of the OrderJournal of virology · 2020Article
- Visualizing Sacbrood Virus of Honey Bees via Transformation and Coupling with Enhanced Green Fluorescent Protein.Viruses · 2020Article
- A Novel Iflavirus Was Discovered in Green Rice LeafhopperFrontiers in microbiology · 2020Article
- Review
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Infection by sacbrood virus (SBV) from the family Iflaviridae is lethal to honey bee larvae but only rarely causes the collapse of honey bee colonies. Despite the negative effect of SBV on honey bees, the structure of its particles and mechanism of its genome delivery are unknown. Here we present the crystal structure of SBV virion and show that it contains 60 copies of a minor capsid protein (MiCP) attached to the virion surface. No similar MiCPs have been previously reported in any of the related viruses from the order Picornavirales. The location of the MiCP coding sequence within the SBV genome indicates that the MiCP evolved from a C-terminal extension of a major capsid protein by the introduction of a cleavage site for a virus protease. The exposure of SBV to acidic pH, which the virus likely encounters during cell entry, induces the formation of pores at threefold and fivefold axes of the capsid that are 7 Å and 12 Å in diameter, respectively. This is in contrast to vertebrate picornaviruses, in which the pores along twofold icosahedral symmetry axes are currently considered the most likely sites for genome release. SBV virions lack VP4 subunits that facilitate the genome delivery of many related dicistroviruses and picornaviruses. MiCP subunits induce liposome disruption in vitro, indicating that they are functional analogs of VP4 subunits and enable the virus genome to escape across the endosome membrane into the cell cytoplasm.
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