Evidence map›Paper›PMID 29986717›Full record

ArticleMalaria journal2018

Evaluation of loop-mediated isothermal amplification as a surveillance tool for malaria in reactive case detection moving towards elimination.

Munyaradzi Tambo, Joyce R Auala, Hugh J Sturrock, Immo Kleinschmidt, Ronnie Bock, Jennifer L Smith, Roland Gosling, Davis R Mumbengegwi

Open access · goldAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Malaria journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed, 2 pooled it
2.3field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 2 syntheses or guidelines pooled it, 18 citations in OpenAlex.

  1. Pooled it
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  6. Current methods for the detection ofCurrent research in parasitology & vector-borne diseases · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 3 countries.

Munyaradzi TamboMultidisciplinary Research Center, University of Namibia, Windhoek, Namibia.
Joyce R AualaMultidisciplinary Research Center, University of Namibia, Windhoek, Namibia.
Hugh J SturrockMalaria Elimination Initiative, Global Health Group, University of California San Francisco, San Francisco, CA, USA.
Immo KleinschmidtMRC Tropical Epidemiology Group, Department of Infectious Disease Epidemiology, London School of Hygiene and Tropical Medicine, London, UK.
Ronnie BockDepartment of Biological Sciences, University of Namibia, Windhoek, Namibia.
Jennifer L SmithMalaria Elimination Initiative, Global Health Group, University of California San Francisco, San Francisco, CA, USA.
Roland GoslingMalaria Elimination Initiative, Global Health Group, University of California San Francisco, San Francisco, CA, USA.
Davis R MumbengegwiMultidisciplinary Research Center, University of Namibia, Windhoek, Namibia. dmumbengegwi@unam.na.
University of Namibia · NAUniversity of California, San Francisco · USLondon School of Hygiene & Tropical Medicine · GB

Funding

Medical Research Council MR/K012126/1
6 · The paper itself

Abstract

backgroundAs malaria transmission decreases, the proportion of infections that are asymptomatic at any given time increases. This poses a challenge for diagnosis as routinely used rapid diagnostic tests (RDTs) miss asymptomatic malaria cases with low parasite densities due to poor sensitivity. Yet, asymptomatic infections can contribute to onward transmission of malaria and therefore act as infectious reservoirs and perpetuate malaria transmission. This study compared the performance of RDTs to loop-mediated isothermal amplification (LAMP) in the diagnosis of malaria during reactive active case detection surveillance.

methodsAll reported malaria cases in the Engela Health District of Namibia were traced back to their place of residence and persons living within the four closest neighbouring houses to the index case (neighbourhood) were tested for malaria infection with RDTs and dried blood spots (DBS) were collected. LAMP and nested PCR (nPCR) were carried out on all RDTs and DBS. The same procedure was followed in randomly selected control neighbourhoods.

resultsSome 3151 individuals were tested by RDT, LAMP and nPCR. Sensitivity of RDTs and LAMP were 9.30 and 95.50%, respectively, and specificities were 99.27 and 99.92%, respectively, compared to nPCR. LAMP carried out on collected RDTs showed a sensitivity and specificity of 95.35 and 99.85% compared to nPCR carried out on DBS. There were 2 RDT samples that were negative by LAMP but the corresponding DBS samples were positive by PCR.

conclusionThe study showed that LAMP had the equivalent performance as nPCR for the identification of Plasmodium falciparum infection. Given its relative simplicity to implement over more complex and time-consuming methods, such as PCR, LAMP is particularly useful in elimination settings where high sensitivity and ease of operation are important.

Indexed as

Disease EradicationDiagnostic Tests, RoutineMalaria, FalciparumNamibiaNucleic Acid Amplification TechniquesPlasmodium falciparumPolymerase Chain ReactionPopulation SurveillanceSensitivity and Specificity

Identifiers

PMID29986717
PMCPMC6038281
OpenAlexW2865044057

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.