ArticleNucleic acids research2018
Engineering orthogonal synthetic timer circuits based on extracytoplasmic function σ factors.
Article in Nucleic acids research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Mixed positive and negative feedback loops drive diverse single-cell gene expression dynamics.Nature communications · 2026Article
- Tuning and functionalization of logic gates for time resolved programming of bacterial populations.Nucleic acids research · 2025Article
- Toward predictable universal genetic circuit design.Quantitative biology (Beijing, China) · 2024Article
- A MoClo-Compatible Toolbox of ECF Sigma Factor-Based Regulatory Switches for Proteobacterial Chassis.Biodesign research · 2024Article
- Customizing cellular signal processing by synthetic multi-level regulatory circuits.Nature communications · 2023Review
- Structure of the transcription open complex of distinct σNature communications · 2023Article
- The σB alternative sigma factor circuit modulates noise to generate different types of pulsing dynamics.PLoS computational biology · 2023Article
- Novel switchable ECF sigma factor transcription system for improving thaxtomin A production inSynthetic and systems biotechnology · 2022Article
- Tunable phenotypic variability through an autoregulatory alternative sigma factor circuit.Molecular systems biology · 2021Article
- A tunable population timer in multicellular consortia.iScience · 2021Article
- Development of a novel heterologous β-lactam-specific whole-cell biosensor inJournal of biological engineering · 2020Article
- The Epipeptide YydF Intrinsically Triggers the Cell Envelope Stress Response ofFrontiers in microbiology · 2020Article
- Where to begin? Sigma factors and the selectivity of transcription initiation in bacteria.Molecular microbiology · 2019Review
- Alternative σI/anti-σI factors represent a unique form of bacterial σ/anti-σ complex.Nucleic acids research · 2019Article
- Diversity, versatility and complexity of bacterial gene regulation mechanisms: opportunities and drawbacks for applications in synthetic biology.FEMS microbiology reviews · 2019Review
- Extracytoplasmic Function σ Factors Can Be Implemented as Robust Heterologous Genetic Switches in Bacillus subtilis.iScience · 2019Article
- Article
- CRIMoClo plasmids for modular assembly and orthogonal chromosomal integration of synthetic circuits inJournal of biological engineering · 2019Article
- MostmSphere · 2018Article
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6 authors.
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Abstract
The rational design of synthetic regulatory circuits critically hinges on the availability of orthogonal and well-characterized building blocks. Here, we focus on extracytoplasmic function (ECF) σ factors, which are the largest group of alternative σ factors and hold extensive potential as synthetic orthogonal regulators. By assembling multiple ECF σ factors into regulatory cascades of varying length, we benchmark the scalability of the approach, showing that these 'autonomous timer circuits' feature a tuneable time delay between inducer addition and target gene activation. The implementation of similar timers in Escherichia coli and Bacillus subtilis shows strikingly convergent circuit behavior, which can be rationalized by a computational model. These findings not only reveal ECF σ factors as powerful building blocks for a rational, multi-layered circuit design, but also suggest that ECF σ factors are universally applicable as orthogonal regulators in a variety of bacterial species.
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