ArticleNature biotechnology2018
A genetically encoded fluorescent acetylcholine indicator for in vitro and in vivo studies.
Article in Nature biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 209 papers.
What it found
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Who cites it
209 citing papers in PubMed, 402 citations in OpenAlex.
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- The CAGE Framework (Calcium-Gated Excitability): From Single Neurotransmitters to Calcium States in the Tumor Microenvironment.International journal of molecular sciences · 2026Review
- Red-shifted GRAB acetylcholine sensors for multiplex imaging in vivo.Nature neuroscience · 2026Article
- Optical insights into spatial precision and release heterogeneity of neuromodulatory transmission.iScience · 2026Article
- Ratiometric iGluSnFr imaging to assess tonic glutamate in the cerebral cortex.bioRxiv : the preprint server for biology · 2026Article
- Quantitative anatomy and biophysical modeling of ascending neuromodulatory systems in the developing rat neocortex.PLoS computational biology · 2026Article
- Spatiotemporally dynamic noradrenergic regulation of cortical networks.bioRxiv : the preprint server for biology · 2026Article
- A Novel Acetylcholine Nanosensor for Single Vesicle Storage and Sub-Quantal Exocytosis in Living Neurons and Organoids.Angewandte Chemie (International ed. in English) · 2026Article
- The neurovascular impulse response function differentially reflects intrinsic neuromodulation across cortical regions.Nature neuroscience · 2026Article
- Dynamic neuronal ensembles encode burst-suppression revealed by cortex-wide optical-electrical interfaces.Nature communications · 2026Article
- Lymphocyte-derived cholinergic circuits modulate germinal center output and B cell activation.Nature immunology · 2026Article
- AQuA2-Cloud: a web platform for fluorescence bioimaging activity analysis.bioRxiv : the preprint server for biology · 2026Article
- In vivo multimodal neurochemical interfaces for real-time decoding of brain circuit.Nature reviews. Neuroscience · 2026Review
- Light-Based Molecular Tools to Precisely Monitor and Operate β-Adrenoceptors.Medicinal research reviews · 2026Review
- FLIPs: Genetically encoded molecular biosensors for functional imaging of cell signaling by linear dichroism microscopy.Science advances · 2026Article
- A high-throughput screening platform for acetylcholinesterase inhibitors using a genetically encoded acetylcholine fluorescent sensor.Frontiers in bioengineering and biotechnology · 2026Article
- Live-cell physiology in human brain tissue culture-the potential, the challenges, and the lessons learned.Frontiers in cellular neuroscience · 2026Review
- Neurotransmitter co-transmission: synaptic architectures, functional logic, and emerging tools.Frontiers in molecular neuroscience · 2026Review
149 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
21 authors at 9 institutions in 3 countries.
Funding
Abstract
The neurotransmitter acetylcholine (ACh) regulates a diverse array of physiological processes throughout the body. Despite its importance, cholinergic transmission in the majority of tissues and organs remains poorly understood owing primarily to the limitations of available ACh-monitoring techniques. We developed a family of ACh sensors (GACh) based on G-protein-coupled receptors that has the sensitivity, specificity, signal-to-noise ratio, kinetics and photostability suitable for monitoring ACh signals in vitro and in vivo. GACh sensors were validated with transfection, viral and/or transgenic expression in a dozen types of neuronal and non-neuronal cells prepared from multiple animal species. In all preparations, GACh sensors selectively responded to exogenous and/or endogenous ACh with robust fluorescence signals that were captured by epifluorescence, confocal, and/or two-photon microscopy. Moreover, analysis of endogenous ACh release revealed firing-pattern-dependent release and restricted volume transmission, resolving two long-standing questions about central cholinergic transmission. Thus, GACh sensors provide a user-friendly, broadly applicable tool for monitoring cholinergic transmission underlying diverse biological processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.