Evidence map›Paper›PMID 29985456›Full record

Trial reportSpinal cord2018

Normalization of disrupted clock gene expression in males with tetraplegia: a crossover randomized placebo-controlled trial of melatonin supplementation.

Emil Kostovski, Elena Frigato, Mladen Savikj, Anders Dahm, Per Morten Sandset, Marie-Christine Mowinckel, Grethe Skretting, Bjarne Østerud, Cristiano Bertolucci, Per Ole Iversen

Open access · bronzeAbstract readRandomized Controlled Trial
PubMed Publisher
In one paragraph

Trial report in Spinal cord, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.8field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 13 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 2 countries.

Emil KostovskiDepartment of Research, Sunnaas Rehabilitation Hospital, Nesoddtangen, Norway. Emil.Kostovski@sunnaas.no.ORCID http://orcid.org/0000-0002-7037-1363
Elena FrigatoDepartment of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy.
Mladen SavikjDepartment of Research, Sunnaas Rehabilitation Hospital, Nesoddtangen, Norway.
Anders DahmFaculty of Medicine, University of Oslo, Oslo, Norway.
Per Morten SandsetFaculty of Medicine, University of Oslo, Oslo, Norway.
Marie-Christine MowinckelDepartment of Haematology, Oslo University Hospital, Oslo, Norway.
Grethe SkrettingDepartment of Haematology, Oslo University Hospital, Oslo, Norway.
Bjarne ØsterudFaculty of Medicine, University of Tromsø, Tromsø, Norway.
Cristiano BertolucciDepartment of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy.
Per Ole IversenDepartment of Haematology, Oslo University Hospital, Oslo, Norway.
Oslo University Hospital · NOUniversity of Ferrara · ITUniversity of Oslo · NOUiT The Arctic University of Norway · NO

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

STUDY

designCrossover double blind, randomized placebo-controlled trial.

objectivesCircadian oscillators are located both in the brain and in peripheral organs. Melatonin, the main brain-derived hormone governing circadian variations, is highly associated with daylight patterns. However, in subjects with tetraplegia the melatonin levels are blunted. Here we studied peripheral oscillators in peripheral blood mononuclear cells (PBMCs) in males with tetraplegia by examining how exogenous melatonin may influence the expression of clock gene mRNAs.

settingSunnaas Rehabilitation Hospital, Nesoddtangen, Norway.

methodsSix males with tetraplegia received 2 mg of melatonin or placebo 4 days before the study period. We also included six able-bodied men sleeping or kept awake during the night. Plasma samples were collected four times during a 24-h period. The mRNA expression levels of the clock genes PER1, PER2, BMAL1, and REV-ERBα were quantified in PBMCs using quantitative RT-PCR.

resultsThe mRNA expression levels of PER-1 and -2 and REV-ERBα were increased at 04:00 h compared with the able-bodied controls (p < 0.05). Melatonin supplementation changed mRNA peak-time toward the time of supplementation.

conclusionsSeveral peripheral clock genes displayed distorted expression levels in tetraplegia. Supplementation with melatonin changed the mRNA expression levels of these genes toward those observed among able-bodied. SPONSORSHIP: Financial support was provided from the Throne Holst Foundation, Sunnaas Rehabilitation hospital and the University of Ferrara (FAR2016).

Indexed as

AdultCentral Nervous System AgentsCLOCK ProteinsCross-Over StudiesDouble-Blind MethodHumansLeukocytes, MononuclearMaleMelatoninMiddle AgedQuadriplegiaRNA, MessengerSleep DeprivationCentral Nervous System AgentsCLOCK ProteinsMelatoninRNA, Messenger

Identifiers

PMID29985456
OpenAlexW2836333704

What OpenQuestion holds

Textmetadata
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.