ArticleJournal of visualized experiments : JoVE2018
Methods for the Study of Regeneration in Stentor.
Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 20 citations in OpenAlex.
- A role for theMolecular biology of the cell · 2026Article
- Spatiotemporal control of cortical centrin patterning by regionalized Sfi1 family scaffolding proteins in Stentor coeruleus.Current biology : CB · 2026Article
- Genome-wide analysis of mRNA regionalization in a giant single cell.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Stentor stipatus is a new unicellular species that demonstrates habituation and unique phototaxis.Scientific reports · 2026Article
- A role for thebioRxiv : the preprint server for biology · 2025Article
- A receptor-inactivation model for single-celled habituation in Stentor coeruleus.Current biology : CB · 2025Article
- Characterization of cellular wound resistance in the giant ciliatebioRxiv : the preprint server for biology · 2025Article
- The cell biology and genome ofMolecular biology of the cell · 2025Article
- Genome-wide analysis of anterior-posterior mRNA regionalization inbioRxiv : the preprint server for biology · 2024Article
- Phylogeny, morphology, and behavior of the new ciliate speciesbioRxiv : the preprint server for biology · 2024Article
- SMORES: a simple microfluidic operating room for the examination and surgery of Stentor coeruleus.Scientific reports · 2024Article
- SMORES: A Simple Microfluidic Operating Room for the Examination and Surgery ofbioRxiv : the preprint server for biology · 2024Article
- The nuclear transport factor CSE1 drives macronuclear volume increase and macronuclear node coalescence iniScience · 2023Article
- Single-cell analysis of habituation in Stentor coeruleus.Current biology : CB · 2023Article
- Studying Habituation in Stentor coeruleus.Journal of visualized experiments : JoVE · 2023Article
- Article
- Determining protein polarization proteome-wide using physical dissection of individual Stentor coeruleus cells.Current biology : CB · 2022Article
- Exercise, Diet and Sleeping as Regenerative Medicine Adjuvants: Obesity and Ageing as Illustrations.Medicines (Basel, Switzerland) · 2022Review
- Analysis of Motility Patterns of Stentor During and After Oral Apparatus Regeneration Using Cell Tracking.Journal of visualized experiments : JoVE · 2021Article
- Microfluidic guillotine reveals multiple timescales and mechanical modes of wound response in Stentor coeruleus.BMC biology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Cells need to be able to regenerate their parts to recover from external perturbations. The unicellular ciliate Stentor coeruleus is an excellent model organism to study wound healing and subsequent cell regeneration. The Stentor genome became available recently, along with modern molecular biology methods, such as RNAi. These tools make it possible to study single-cell regeneration at the molecular level. The first section of the protocol covers establishing Stentor cell cultures from single cells or cell fragments, along with general guidelines for maintaining Stentor cultures. Culturing Stentor in large quantities allows for the use of valuable tools like biochemistry, sequencing, and mass spectrometry. Subsequent sections of the protocol cover different approaches to inducing regeneration in Stentor. Manually cutting cells with a glass needle allows studying the regeneration of large cell parts, while treating cells with either sucrose or urea allows studying the regeneration of specific structures located at the anterior end of the cell. A method for imaging individual regenerating cells is provided, along with a rubric for staging and analyzing the dynamics of regeneration. The entire process of regeneration is divided in three stages. By visualizing the dynamics of the progression of a population of cells through the stages, the heterogeneity in regeneration timing is demonstrated.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.