ArticleMicroRNA (Shariqah, United Arab Emirates)2018
The Influence of Pre-Analytical Factors on the Analysis of Circulating MicroRNA.
Article in MicroRNA (Shariqah, United Arab Emirates), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Differences in serum and plasma levels of microRNAs and their time-course changes after blood collection.Practical laboratory medicine · 2024Article
- Analysis of the longitudinal stability of human plasma miRNAs and implications for disease biomarkers.Scientific reports · 2024Article
- False Positives in Brucellosis Serology: Wrong Bait and Wrong Pond?Tropical medicine and infectious disease · 2023Review
- MicroRNAs: Emerging as Highly Promising Biomarkers for Early Breast Cancer Screening.Journal of clinical and laboratory medicine · 2023Article
- Circulating microRNAs: Challenges with their use as liquid biopsy biomarkers.Cancer biomarkers : section A of Disease markers · 2022Review
- Comparative Analyses of Liquid-Biopsy MicroRNA371a-3p Isolation Protocols for Serum and Plasma.Cancers · 2021Article
- Epitranscriptomics of Ischemic Heart Disease-The IHD-EPITRAN Study Design and Objectives.International journal of molecular sciences · 2021Article
- Methodological considerations for measuring biofluid-based microRNA biomarkers.Critical reviews in toxicology · 2021Article
- Circulating exosomal microRNAs as novel potential detection biomarkers in pancreatic cancer.Oncology letters · 2020Article
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMicroRNAs (miRNA) are expected as useful biomarkers for various diseases. We studied the pre-analytical factors causing variation in the analysis of miRNA. MATERIAL AND
methodsBlood samples were collected from 25 healthy subjects. Plasma and serum were obtained from the same samples. The levels of miR-451, -16, -126, and -223 were analyzed using RT-qPCR. Cel-miR-39 was added as a spiked-in control in each sample.
resultsWith the exception of miR-451, the levels of the miRNAs in plasma were higher than in serum. After high-speed centrifugation, the levels of miRNAs were almost equal between plasma and serum except for miR-451. Membrane filtration with 0.45 µm pore size reduced the levels of plasma miRNAs. The coagulation accelerators for serum processing did not affect the analysis of miRNA. The use of fraction containing particles of > 0.45 µm in size showed the inhibitory effect on the analysis of plasma miR-451. The RNase inhibitor was effective for protecting against the degradation of miRNAs.
conclusionPlasma contains factors modifying miRNA profiles. The immediate processing of plasma with membrane filtration and RNase inhibitor may be a relevant method for achieving the stable analysis of miRNA.
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