ArticleMicrobial biotechnology2018
Stepwise modifications of genetic parts reinforce the secretory production of nattokinase in Bacillus subtilis.
Article in Microbial biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- Engineered Bacillus subtilis for high-yield nattokinase production and therapeutic potential in obesity management.Journal of animal science and biotechnology · 2026Article
- Development of Bacillus subtilis cell factories for nutraceuticals production.World journal of microbiology & biotechnology · 2026Review
- Genome report of a probioticMicrobiology resource announcements · 2025Article
- Research Progress of Nattokinase in Reducing Blood Lipid.Nutrients · 2025Review
- Genomic and Transcriptomic Analysis of MutantFoods (Basel, Switzerland) · 2025Article
- Computational analysis to comprehend the structure-function properties of fibrinolytic enzymes fromHeliyon · 2024Article
- Advances in recombinant protease production: current state and perspectives.World journal of microbiology & biotechnology · 2024Review
- High and Economical Nattokinase Production with Acetoin as a Useful Byproduct from Soybean Milk and Glucose.Probiotics and antimicrobial proteins · 2022Article
- Development and application of a rapid all-in-one plasmid CRISPR-Cas9 system for iterative genome editing in Bacillus subtilis.Microbial cell factories · 2022Article
- Analysis of Cross-Functionality within LanBTC Synthetase Complexes from Different Bacterial Sources with Respect to Production of Fully Modified Lanthipeptides.Applied and environmental microbiology · 2022Article
- Development of a novel strategy for robust synthetic bacterial promoters based on a stepwise evolution targeting the spacer region of the core promoter in Bacillus subtilis.Microbial cell factories · 2019Article
- Efficient production of extracellular pullulanase in Bacillus subtilis ATCC6051 using the host strain construction and promoter optimization expression system.Microbial cell factories · 2018Article
- Exploitation of Bacillus subtilis as a robust workhorse for production of heterologous proteins and beyond.World journal of microbiology & biotechnology · 2018Review
- Stepwise modifications of genetic parts reinforce the secretory production of nattokinase in Bacillus subtilis.Microbial biotechnology · 2018Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nattokinase (NK) is an important serine-protease with direct fibrinolytic activity involving the prevention of cardiovascular disease as an antithrombotic agent. Dozens of studies have focused on the characterization of intrinsic novel promoters and signal peptides to the secretory production of recombinant proteins in Bacillus subtilis. However, intrinsic genetic elements have several drawbacks, which cannot mediate the production of NK to the desired level. In this study, the genetic elements, which were used to overproduce the recombinant secretory NK, were rationally modified in B. subtilis in a stepwise manner. The first step was to select a suitable signal peptide for the highly efficient secretion of NK. By comparison of the secretory levels mediated by two different signal peptides, which were encoded by the genes of a minor extracellular protease epr (SP
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