Evidence map›Paper›PMID 29984109›Full record

ArticleBiomedical optics express2018

Real-time visualization of two-photon fluorescence lifetime imaging microscopy using a wavelength-tunable femtosecond pulsed laser.

Jiheun Ryu, Ungyo Kang, Jayul Kim, Hyunjun Kim, Jue Hyung Kang, Hyunjin Kim, Dae Kyung Sohn, Jae-Heon Jeong, Hongki Yoo, Bomi Gweon

Abstract read
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In one paragraph

Article in Biomedical optics express, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed.

  1. Article
  2. Article
  3. Fluorescence Lifetime Imaging Microscopy Analysis of Isolated Melanosomes.Chemphyschem : a European journal of chemical physics and physical chemistry · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Jiheun RyuDepartment of Mechanical Engineering, Korea Advanced Institute of Science and Technology, Daejeon 34141, South Korea.
Ungyo KangDepartment of Biomedical Engineering, Hanyang University, Seoul 04763, South Korea.
Jayul KimDepartment of Mechanical Engineering, Korea Advanced Institute of Science and Technology, Daejeon 34141, South Korea.
Hyunjun KimDepartment of Mechanical Engineering, Korea Advanced Institute of Science and Technology, Daejeon 34141, South Korea.
Jue Hyung KangDepartment of Biomedical Engineering, Hanyang University, Seoul 04763, South Korea.
Hyunjin KimMolecular Imaging & Therapy Branch, Research Institute and Hospital, National Cancer Center, Goyang, 10408, South Korea.
Dae Kyung SohnInnovative Medical Engineering & Technology, Division of Convergence Technology, Research Institute and Hospital, National Cancer Center, Goyang, 10408, South Korea.
Jae-Heon JeongDepartment of Mechanical Engineering, Korea Advanced Institute of Science and Technology, Daejeon 34141, South Korea.
Hongki YooDepartment of Biomedical Engineering, Hanyang University, Seoul 04763, South Korea.
Bomi GweonDepartment of Biomedical Engineering, Hanyang University, Seoul 04763, South Korea.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A fluorescence lifetime imaging microscopy (FLIM) integrated with two-photon excitation technique was developed. A wavelength-tunable femtosecond pulsed laser with nominal pulse repetition rate of 76-MHz was used to acquire FLIM images with a high pixel rate of 3.91 MHz by processing the pulsed two-photon fluorescence signal. Analog mean-delay (AMD) method was adopted to accelerate the lifetime measurement process and to visualize lifetime map in real-time. As a result, rapid tomographic visualization of both structural and chemical properties of the tissues was possible with longer depth penetration and lower photo-damage compared to the conventional single-photon FLIM techniques.

Indexed as

(110.6880) Three-dimensional image acquisition(170.3650) Lifetime-based sensing(180.2520) Fluorescence microscopy(180.4315) Nonlinear microscopy

Identifiers

PMID29984109
PMCPMC6033550

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.