ArticleBiomedical optics express2018
On-chip light-sheet fluorescence imaging flow cytometry at a high flow speed of 1 m/s.
Article in Biomedical optics express, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- An Integrated Micromirror System for High-Throughput Multi-Parametric Cell Imaging.Small methods · 2026Article
- Editorial: Multiomics advances: bridging gaps in understanding intricate biological systems.Frontiers in plant science · 2026Article
- Integrated microoptical system for continuous fluorescence monitoring of microtissues.Microsystems & nanoengineering · 2025Article
- Next-Generation Biomaterials: Design Strategies, Clinical Translation, and the Rise of Intelligent Therapeutic Platforms.ACS omega · 2025Review
- Femtosecond laser microfabrication of a fully-integrated optofluidic device for 3D imaging flow cytometry.Scientific reports · 2025Article
- Review
- Multicolor iLIFE (m-iLIFE) volume cytometry for high-throughput imaging of multiple organelles.Scientific reports · 2024Article
- Recent advances in oblique plane microscopy.Nanophotonics (Berlin, Germany) · 2023Review
- Large-scale high-throughput 3D culture, imaging, and analysis of cell spheroids using microchip-enhanced light-sheet microscopy.Biomedical optics express · 2023Article
- Flow Cytometry with Anti-Diffraction Light Sheet (ADLS) by Spatial Light Modulation.Micromachines · 2023Article
- Portable light-sheet optofluidic microscopy for 3D fluorescence imaging flow cytometry.Lab on a chip · 2023Article
- Single-Lens Light-Sheet Fluorescence Microscopy Based on Micro-Mirror Array.Laser & photonics reviews · 2022Article
- Modular multimodal platform for classical and high throughput light sheet microscopy.Scientific reports · 2022Article
- High-speed fluorescence image-enabled cell sorting.Science (New York, N.Y.) · 2022Article
- Light sheet based volume flow cytometry (VFC) for rapid volume reconstruction and parameter estimation on the go.Scientific reports · 2022Article
- Single-objective high-resolution confocal light sheet fluorescence microscopy for standard biological sample geometries.Biomedical optics express · 2021Article
- High-throughput 3D imaging of single cells with light-sheet fluorescence microscopy on chip.Biomedical optics express · 2020Article
- A practical guide to intelligent image-activated cell sorting.Nature protocols · 2019Article
- Applications of Light-Sheet Microscopy in Microdevices.Frontiers in neuroanatomy · 2019Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present on-chip fluorescence imaging flow cytometry by light-sheet excitation on a mirror-embedded microfluidic chip. The method allows us to obtain microscopy-grade fluorescence images of cells flowing at a high speed of 1 m/s, which is comparable to the flow speed of conventional non-imaging flow cytometers. To implement the light-sheet excitation of flowing cells in a microchannel, we designed and fabricated a mirror-embedded PDMS-based microfluidic chip. To show its broad utility, we used the method to classify large populations of microalgal cells (
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