ArticleThe Plant journal : for cell and molecular biology2018
Subdiffraction-resolution live-cell imaging for visualizing thylakoid membranes.
Article in The Plant journal : for cell and molecular biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 55 citations in OpenAlex.
- Specific light-harvesting complexes mediate grana stacking and prevent energy spillover between photosystems in plant chloroplasts.The Plant cell · 2026Article
- State transitions in Physcomitrium patens studied with time-resolved fluorescence.Photosynthesis research · 2026Article
- What are grana in chloroplasts of vascular plants good for?Annals of botany · 2026Review
- Charge reversal at the Lhcb2 N-terminus impairs phosphorylation and PSI-LHCII complex formation.Photosynthesis research · 2026Article
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- Too dim, too bright, and just right: Systems analysis of the Chlamydomonas diurnal program under limiting and excess light.The Plant cell · 2025Article
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- De-etiolation is Almost Color Blind: The Study of Photosynthesis Awakening under Blue and Red Light.Plant & cell physiology · 2024Article
- Macroscale structural changes of thylakoid architecture during high light acclimation in Chlamydomonas reinhardtii.Photosynthesis research · 2024Article
- Manganese concentration affects chloroplast structure and the photosynthetic apparatus in Marchantia polymorpha.Plant physiology · 2023Article
- Action of 2,6-Dichloro-1,4-benzoquinone on the OCells · 2023Article
- Single chloroplast in folio imaging sheds light on photosystem energy redistribution during state transitions.Plant physiology · 2023Article
- Microdomains heterogeneity in the thylakoid membrane proteins visualized by super-resolution microscopy.Photosynthetica · 2023Article
- State transition is quiet around pyrenoid and LHCII phosphorylation is not essential for thylakoid deformation inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- How to Measure Grana - Ultrastructural Features of Thylakoid Membranes of Plant Chloroplasts.Frontiers in plant science · 2021Review
- A brief history of how microscopic studies led to the elucidation of the 3D architecture and macromolecular organization of higher plant thylakoids.Photosynthesis research · 2020Review
- Regulation of Light Harvesting inPlant physiology · 2020Article
- Modeling the Role of LHCII-LHCII, PSII-LHCII, and PSI-LHCII Interactions in State Transitions.Biophysical journal · 2020Article
- The Main Structural and Functional Characteristics of Photosystem-II-Enriched Membranes Isolated from Wild Type andLife (Basel, Switzerland) · 2020Article
- Atomic Force Microscopy Visualizes Mobility of Photosynthetic Proteins in Grana Thylakoid Membranes.Biophysical journal · 2020Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The chloroplast is the chlorophyll-containing organelle that produces energy through photosynthesis. Within the chloroplast is an intricate network of thylakoid membranes containing photosynthetic membrane proteins that mediate electron transport and generate chemical energy. Historically, electron microscopy (EM) has been a powerful tool for visualizing the macromolecular structure and organization of thylakoid membranes. However, an understanding of thylakoid membrane dynamics remains elusive because EM requires fixation and sectioning. To improve our knowledge of thylakoid membrane dynamics we need to consider at least two issues: (i) the live-cell imaging conditions needed to visualize active processes in vivo; and (ii) the spatial resolution required to differentiate the characteristics of thylakoid membranes. Here, we utilize three-dimensional structured illumination microscopy (3D-SIM) to explore the optimal imaging conditions for investigating the dynamics of thylakoid membranes in living plant and algal cells. We show that 3D-SIM is capable of examining broad characteristics of thylakoid structures in chloroplasts of the vascular plant Arabidopsis thaliana and distinguishing the structural differences between wild-type and mutant strains. Using 3D-SIM, we also visualize thylakoid organization in whole cells of the green alga Chlamydomonas reinhardtii. These data reveal that high light intensity changes thylakoid membrane structure in C. reinhardtii. Moreover, we observed the green alga Chromochloris zofingiensis and the moss Physcomitrella patens to show the applicability of 3D-SIM. This study demonstrates that 3D-SIM is a promising approach for studying the dynamics of thylakoid membranes in photoautotrophic organisms during photoacclimation processes.
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