ArticleNucleic acids research2018
Generating genomic platforms to study Candida albicans pathogenesis.
Article in Nucleic acids research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- β-1,6-Glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wall.eLife · 2024Article
- Metabolic reprogramming during Candida albicans planktonic-biofilm transition is modulated by the transcription factors Zcf15 and Zcf26.PLoS biology · 2024Article
- A gain-of-function mutation in zinc cluster transcription factor Rob1 drives Candida albicans adaptive growth in the cystic fibrosis lung environment.PLoS pathogens · 2024Article
- GenoPipe: identifying the genotype of origin within (epi)genomic datasets.Nucleic acids research · 2023Article
- Development and applications of a CRISPR activation system for facile genetic overexpression in Candida albicans.G3 (Bethesda, Md.) · 2023Article
- Candida albicans commensalism in the oral mucosa is favoured by limited virulence and metabolic adaptation.PLoS pathogens · 2022Article
- Overexpression approaches to advance understanding of Candida albicans.Molecular microbiology · 2022Review
- Functional Portrait of Irf1 (Orf19.217), a Regulator of Morphogenesis and Iron Homeostasis inFrontiers in cellular and infection microbiology · 2022Article
- ChIP-SICAP: A New Tool to Explore Gene-Regulatory Networks in Candida albicans and Other Yeasts.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Hsp90 interaction networks in fungi-tools and techniques.FEMS yeast research · 2021Review
- Genetic Manipulation as a Tool to UnravelJournal of fungi (Basel, Switzerland) · 2021Review
- A conserved regulator controls asexual sporulation in the fungal pathogen Candida albicans.Nature communications · 2020Article
- Use of CRISPR-Cas9 To Target Homologous Recombination Limits Transformation-Induced Genomic Changes in Candida albicans.mSphere · 2020Article
- The Transcription Factor Stp2 Is Important forFrontiers in microbiology · 2020Article
- Recent advances in understandingF1000Research · 2019Review
- Protein-Protein Interactions inFrontiers in microbiology · 2019Review
- Systematic gene overexpression in Candida albicans identifies a regulator of early adaptation to the mammalian gut.Cellular microbiology · 2018Article
- A High-ThroughputmSphere · 2018Article
Corrections and comments
- Erratum issued
Authors and funding
26 authors at 4 institutions in 3 countries.
Funding
Abstract
The advent of the genomic era has made elucidating gene function on a large scale a pressing challenge. ORFeome collections, whereby almost all ORFs of a given species are cloned and can be subsequently leveraged in multiple functional genomic approaches, represent valuable resources toward this endeavor. Here we provide novel, genome-scale tools for the study of Candida albicans, a commensal yeast that is also responsible for frequent superficial and disseminated infections in humans. We have generated an ORFeome collection composed of 5099 ORFs cloned in a Gateway™ donor vector, representing 83% of the currently annotated coding sequences of C. albicans. Sequencing data of the cloned ORFs are available in the CandidaOrfDB database at http://candidaorfeome.eu. We also engineered 49 expression vectors with a choice of promoters, tags and selection markers and demonstrated their applicability to the study of target ORFs transferred from the C. albicans ORFeome. In addition, the use of the ORFeome in the detection of protein-protein interaction was demonstrated. Mating-compatible strains as well as Gateway™-compatible two-hybrid vectors were engineered, validated and used in a proof of concept experiment. These unique and valuable resources should greatly facilitate future functional studies in C. albicans and the elucidation of mechanisms that underlie its pathogenicity.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.