ArticleNucleic acids research2018
Identification of a novel tRNA wobble uridine modifying activity in the biosynthesis of 5-methoxyuridine.
Article in Nucleic acids research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 20 citations in OpenAlex.
- Conditions Enabling the Persistence of Cooperating Synthetase, Ligase, and Mutation-Inhibitor Catalytic Polymers.Journal of molecular evolution · 2026Article
- Unconventional monooxygenation by the ONature chemical biology · 2026Article
- Growth-dependent tRNA Reprogramming and Codon Bias Link Translation to Metabolic State inbioRxiv : the preprint server for biology · 2026Article
- In Silico Analysis of Potential Stabilizer Binding Sites at Protein-RNA Interfaces.Computational and structural biotechnology journal · 2026Article
- tRNA modifications as regulators of bacterial virulence and stress responses.PLoS pathogens · 2025Review
- tRNA-modifying enzymes in bacterial stress adaptation.Open biology · 2025Review
- Identification and comparison of protein composition of biofilms in response to EGCG fromBiofilm · 2024Article
- tRNARNA (New York, N.Y.) · 2024Article
- Nanopore Direct RNA Sequencing for Modified Uridine Nucleotides Yields Signals Dependent on the Physical Properties of the Modified Base.Israel journal of chemistry · 2024Article
- Structural basis for the selective methylation of 5-carboxymethoxyuridine in tRNA modification.Nucleic acids research · 2023Article
- Unique anticodon loop conformation with the flipped-out wobble nucleotide in the crystal structure of unbound tRNARNA (New York, N.Y.) · 2021Article
- Functions of Bacterial tRNA Modifications: From Ubiquity to Diversity.Trends in microbiology · 2021Review
- Survey and Validation of tRNA Modifications and Their Corresponding Genes inBiomolecules · 2020Article
- Identification and Characterization of Genes Required for 5-Hydroxyuridine Synthesis in Bacillus subtilis and Escherichia coli tRNA.Journal of bacteriology · 2019Article
- Dual pathways of tRNA hydroxylation ensure efficient translation by expanding decoding capability.Nature communications · 2019Article
- Structural and biochemical characterization of Rv0187, an O-methyltransferase from Mycobacterium tuberculosis.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Derivatives of 5-hydroxyuridine (ho5U), such as 5-methoxyuridine (mo5U) and 5-oxyacetyluridine (cmo5U), are ubiquitous modifications of the wobble position of bacterial tRNA that are believed to enhance translational fidelity by the ribosome. In gram-negative bacteria, the last step in the biosynthesis of cmo5U from ho5U involves the unique metabolite carboxy S-adenosylmethionine (Cx-SAM) and the carboxymethyl transferase CmoB. However, the equivalent position in the tRNA of Gram-positive bacteria is instead mo5U, where the methyl group is derived from SAM and installed by an unknown methyltransferase. By utilizing a cmoB-deficient strain of Escherichia coli as a host and assaying for the formation of mo5U in total RNA isolates with methyltransferases of unknown function from Bacillus subtilis, we found that this modification is installed by the enzyme TrmR (formerly known as YrrM). Furthermore, X-ray crystal structures of TrmR with and without the anticodon stemloop of tRNAAla have been determined, which provide insight into both sequence and structure specificity in the interactions of TrmR with tRNA.
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