Evidence map›Paper›PMID 29981947›Full record

ArticleActa biomaterialia2018

Development of an apoptosis-assisted decellularization method for maximal preservation of nerve tissue structure.

R C Cornelison, S M Wellman, J H Park, S L Porvasnik, Y H Song, R A Wachs, C E Schmidt

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta biomaterialia, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed.

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  13. The journey of decellularized vessel: from laboratory to operating room.Frontiers in bioengineering and biotechnology · 2024
    Review
  14. Review
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  16. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

R C CornelisonMcKetta Department of Chemical Engineering, University of Texas at Austin, Austin, TX 78705, United States; J. Crayton Pruitt Department of Biomedical Engineering, University of Florida, Gainesville, FL 32611, United States.
S M WellmanJ. Crayton Pruitt Department of Biomedical Engineering, University of Florida, Gainesville, FL 32611, United States.
J H ParkJ. Crayton Pruitt Department of Biomedical Engineering, University of Florida, Gainesville, FL 32611, United States.
S L PorvasnikJ. Crayton Pruitt Department of Biomedical Engineering, University of Florida, Gainesville, FL 32611, United States.
Y H SongJ. Crayton Pruitt Department of Biomedical Engineering, University of Florida, Gainesville, FL 32611, United States.
R A WachsJ. Crayton Pruitt Department of Biomedical Engineering, University of Florida, Gainesville, FL 32611, United States. Electronic address: rebecca.wachs@unl.edu.
C E SchmidtJ. Crayton Pruitt Department of Biomedical Engineering, University of Florida, Gainesville, FL 32611, United States. Electronic address: schmidt@bme.ufl.edu.

Funding

3D Acellular Vascular Beds: Characterization and Re-endothelializationR21EB013358 · NIBIB · UNIVERSITY OF TEXAS AT AUSTIN · PI SCHMIDT, CHRISTINE E · 2011 to 2012
$399k
NIBIB NIH HHS R21 EB013358
6 · The paper itself

Abstract

Preservation of tissue structure is often a primary goal when optimizing tissue and organ decellularization methods. Many current protocols nonetheless rely on detergents that aid extraction of cellular components but also damage tissue architecture. It may be more beneficial to leverage an innate cellular process such as apoptosis and promote cell removal without the use of damaging reagents. During apoptosis, a cell detaches from the extracellular matrix, degrades its internal components, and fragments its contents for easier clearance. We have developed a method that leverages this process to achieve tissue decellularization using only mild wash buffers. We have demonstrated that treating peripheral nerve tissue with camptothecin induced both an early marker of apoptosis, cleaved caspase-3 expression, as well as a late stage marker, TUNEL STATEMENT OF SIGNIFICANCE: Tissue decellularization has expanded the ability to generate non-immunogenic organ replacements for a broad range of health applications. Current technologies typically rely on the use of harsh agents for clearing cellular debris, altering the tissue structure and potentially diminishing the pro-regenerative effects. We have developed a method for effectively, yet gently, removing cellular components from peripheral nerve tissue while preserving the native tissue architecture. The novelty of this process is in the induction of programmed cell death - or apoptosis - via a general cytotoxin, thereby enabling antigen clearance using only hypertonic wash buffers. The resulting acellular nerve scaffolds are nearly identical to unprocessed tissue on a microscopic level and elicit low immune responses comparable to an isograft negative control in a model of subcutaneous implantation.

Indexed as

ApoptosisAnimalsBasement MembraneCamptothecinCaspase 3DetergentsDNA FragmentationExtracellular MatrixGlycosaminoglycansMacrophagesMaleNerve TissuePeripheral Nervous SystemRatsRats, Inbred LewRats, Sprague-DawleyCamptothecinCaspase 3DetergentsGlycosaminoglycansApoptosisDecellularizationDetergent-freePeripheral nerve graft

Identifiers

PMID29981947
PMCPMC6126930

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.