ArticlePathology oncology research : POR2019
Quantitative Analysis on Ex Vivo Nonlinear Microscopy Images of Basal Cell Carcinoma Samples in Comparison to Healthy Skin.
Article in Pathology oncology research : POR, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Prostate Transition Zone Fibrosis is Associated with Clinical Progression in the MTOPS Study.The Journal of urology · 2019Trial
- Tumor border delineation in basal cell carcinoma using Fourier analysis.Lasers in medical science · 2026Article
- ChatGPT, Gemini, and Claude in clinical and dermoscopic image analysis of basal cell carcinoma and its common mimickers: A comparative performance analysis.JID innovations : skin science from molecules to population health · 2026Article
- Dermoscopy-guided high-frequency ultrasound: Principles and applications in dermatology.JID innovations : skin science from molecules to population health · 2026Article
- Novel histological observation method using the scattered light of unstained collagen sections.Scientific reports · 2026Article
- Efficacy of 4% 5-Fluorouracil Cream in the Treatment of Actinic Keratoses: A Single-Center Experience.Journal of clinical medicine · 2026Article
- IL-1β enhances susceptibility to atrial fibrillation in mice by acting through resident macrophages and promoting caspase-1 expression.Nature cardiovascular research · 2025Article
- Label-Free Monitoring of Endometrial Cancer Progression Using Multiphoton Microscopy.Annals of biomedical engineering · 2024Article
- QuantifyingBiophotonics discovery · 2024Article
- Diagnosis of Skin Cancer: From the Researcher Bench to the Patient's Bedside.Journal of clinical medicine · 2024Article
- Quantitative collagen analysis using second harmonic generation images for the detection of basal cell carcinoma with ex vivo multiphoton microscopy.Experimental dermatology · 2023Article
- Multidimensional quantitative characterization of the tumor microenvironment by multicontrast nonlinear microscopy.Biomedical optics express · 2022Article
- Non-Invasive Skin Imaging Assessment of Human Stress During Head-Down Bed Rest Using a Portable Handheld Two-Photon Microscope.Frontiers in physiology · 2022Article
- Article
- Quantitative Multispectral Imaging Differentiates Melanoma from Seborrheic Keratosis.Diagnostics (Basel, Switzerland) · 2021Article
- Visualization of Keratin with Diffuse Reflectance and Autofluorescence Imaging and Nonlinear Optical Microscopy in a Rare Keratinopathic Ichthyosis.Sensors (Basel, Switzerland) · 2021Article
- Recent advances in nonlinear microscopy: Deep insights and polarized revelations.The international journal of biochemistry & cell biology · 2021Review
- Nonlinear optical microscopy is a novel tool for the analysis of cutaneous alterations in pseudoxanthoma elasticum.Lasers in medical science · 2020Article
- Efficiency of long-term high-dose intravenous ascorbic acid therapy in locally advanced basal cell carcinoma - a pilot study.Postepy dermatologii i alergologii · 2020Article
- Exploring Collagen Parameters in Pure Special Types of Invasive Breast Cancer.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Basal cell carcinoma (BCC) is the most frequent malignant neoplasm in the Caucasian population. There are several therapeutic options for BCC, but surgical excision is considered gold standard treatment. As BCCs often have poorly defined borders, the clinical assessment of the tumor margins can be challenging. Therefore, there is an increasing demand for efficient in vivo imaging techniques for the evaluation of tumor borders prior to and during surgeries. In the near future, nonlinear microscopy techniques might meet this demand. We measured the two-photon excitation fluorescence (TPEF) signal of nicotinamide adenine dinucleotide hydride (NADH) and elastin and second harmonic generation (SHG) signal of collagen on 10 ex vivo healthy control and BCC skin samples and compared the images by different quantitative image analysis methods. These included integrated optical density (IOD) measurements on TPEF and SHG images and application of fast Fourier transform (FFT), CT-FIRE and CurveAlign algorithms on SHG images to evaluate the collagen structure. In the BCC samples, we found significantly lower IOD of both the TPEF and SHG signals and higher collagen orientation index utilizing FFT. CT-FIRE algorithm revealed increased collagen fiber length and decreased fiber angle while CurveAlign detected higher fiber alignment of collagen fibers in BCC. These results are in line with previous findings which describe pronounced changes in the collagen structure of BCC. In the future, these novel image analysis methods could be integrated in handheld nonlinear microscope systems, for sensitive and specific identification of BCC.
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