ArticleCurrent microbiology2018
Evaluation of Legiolert for Quantification of Legionella pneumophila from Non-potable Water.
Article in Current microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 34 citations in OpenAlex.
- The Contribution of Chemistry to the Detection and Enumeration of Legionella pneumophila in Environmental Water Samples: Experience With the MICA Method.MicrobiologyOpen · 2026Article
- Comparison between Legiolert and real time PCR in the detection of Legionella pneumophila from environmental water samples.PloS one · 2025Article
- Microbial drinking water monitoring now and in the future.Microbial biotechnology · 2024Review
- Four buildings and a flush: Lessons from degraded water quality and recommendations on building water management.Environmental science and ecotechnology · 2024Article
- On-site filtration of large sample volumes improves the detection of opportunistic pathogens in drinking water distribution systems.Applied and environmental microbiology · 2024Article
- Comparison ofACS ES&T water · 2023Article
- Detection of Diverse Sequence Types of Legionella pneumophila by Legiolert Enzymatic-Based Assay and the Development of a Long-Term Storage Protocol.Microbiology spectrum · 2022Article
- Evaluation of a Most Probable Number Method for Detection and Quantification ofPathogens (Basel, Switzerland) · 2022Article
- Confirming the Presence of Legionella pneumophila in Your Water System: A Review of Current Legionella Testing Methods.Journal of AOAC International · 2021Review
- Performance of Legiolert Test vs. ISO 11731 to ConfirmPathogens (Basel, Switzerland) · 2020Article
- Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Legiolert® is a new culture method for quantification of Legionella pneumophila, which is the primary species associated with Legionnaires' disease. The test is based on a most probable number approach, and differs significantly from traditional culture methods by providing results at 7 days, rapid sample preparation and analysis, and objective interpretation of test results. In this study, we compared the performance of Legiolert with the U.S. Centers for Disease Control and Prevention (CDC) method for detection of L. pneumophila from non-potable samples, primarily comprising cooling tower waters. Our results demonstrated no significant difference between Legiolert and the CDC method for quantification of L. pneumophila. However, Legiolert showed a significant increase in sensitivity when water samples containing higher L. pneumophila concentrations were examined. Cooling tower waters often contain non-Legionella organisms (NLO) that interfere with traditional Legionella test methods, and we observed varying degrees of NLO interference on many CDC method plates. In contrast, Legiolert was resistant to NLO interference and produced a very low rate of false-positive results. Collectively, Legiolert is a sensitive and specific method for quantification of L. pneumophila from non-potable water that provides advantages over the CDC method.
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Registered trials
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