Evidence map›Paper›PMID 29980226›Full record

ArticleBMC veterinary research2018

Immunohistochemical staining patterns of alpha-keratins in normal tissues from two reptile species: implications for characterization of squamous cell carcinomas.

Jorge Orós, María López-Yánez, Francisco Rodríguez, Pascual Calabuig, Pedro L Castro

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Jorge OrósDepartment of Morphology, Veterinary Faculty, University of Las Palmas de Gran Canaria, Trasmontaña s/n, 35413, Arucas, Las Palmas, Spain. jorge.oros@ulpgc.es.ORCID http://orcid.org/0000-0002-8346-5393
María López-YánezDepartment of Morphology, Veterinary Faculty, University of Las Palmas de Gran Canaria, Trasmontaña s/n, 35413, Arucas, Las Palmas, Spain.
Francisco RodríguezDepartment of Morphology, Veterinary Faculty, University of Las Palmas de Gran Canaria, Trasmontaña s/n, 35413, Arucas, Las Palmas, Spain.
Pascual CalabuigTafira Wildlife Rehabilitation Centre, Cabildo de Gran Canaria, Tafira Baja, 35017, Las Palmas de Gran Canaria, Spain.
Pedro L CastroDepartment of Morphology, Veterinary Faculty, University of Las Palmas de Gran Canaria, Trasmontaña s/n, 35413, Arucas, Las Palmas, Spain.
Universidad de Las Palmas de Gran Canaria · ES

Funding

MINECO/FEDER project CGL2015-69084-R
6 · The paper itself

Abstract

backgroundCytokeratins with epitopes in common with those of alpha (acidic and basic) mammalian keratins have been immunohistochemically demonstrated in the epidermis of reptiles. However, there are no reports of immunohistochemical staining patterns of alpha-keratins in other tissues from reptiles. Because the epithelial tumours usually retain the keratin patterns of their normal epithelial origin, it is necessary to know in advance these patterns in the major normal epithelia and organs. We used anti-alpha human keratin AE1 and AE3 monoclonal antibodies to study the staining patterns of alpha-keratins in the major normal epithelia and organs from two reptile species [the bearded dragon (Pogona vitticeps) and the loggerhead sea turtle (Caretta caretta)]. We also studied the immunolocalization of alpha-keratins in squamous cell carcinomas (SCCs) in a bearded dragon and two loggerhead turtles.

resultsAcidic alpha-keratin (AE1 positive) was detected in many of the epithelial tissues of the bearded dragons; however, the detection of basic alpha-keratin (AE3 positive) was much more limited. Alpha-keratins were detected in a greater number of tissues of loggerhead turtles compared with those observed in bearded dragons. In the bearded dragon SCC, all layers of the nests of neoplastic cells, including the cornified layer of the keratin pearls, were strongly reactive with the AE1 antibody. However, a weak reactivity using the AE3 antibody was detected in the basal and intermediate layers of these nests. In the cutaneous SCCs of both sea turtles, acidic alpha-keratin was detected in the basal and suprabasal layers, and in all of the invasive neoplastic cords, while basic alpha-keratin was mainly detected in the invasive neoplastic cords. The pattern observed in the metastases in both turtles consisted of immunohistological detection of acidic alpha-keratin in all metastatic foci, and limited or lack of detection of basic alpha-keratin.

conclusionsThis study provides, for the first time, information about the immunohistochemical staining patterns of alpha-keratins in normal tissues from bearded dragons and loggerhead sea turtles, and confirms the usefulness of AE1 and AE3 monoclonal antibodies in these reptile species. The use of these antibodies also contributed to a better characterization of SCCs in these species.

Indexed as

AnimalsAntibodies, MonoclonalCarcinoma, Squamous CellEpidermisIntestinal MucosaKeratinsLizardsMaleSkin NeoplasmsTurtlesAntibodies, MonoclonalKeratinsAlpha-keratinBearded dragonCaretta carettaImmunohistochemistryLoggerhead Sea turtlePogona vitticepsReptileSquamous cell carcinoma

Identifiers

PMID29980226
PMCPMC6035408
OpenAlexW2822846642

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.