ArticleBiomaterials2018
Studying biomineralization pathways in a 3D culture model of breast cancer microcalcifications.
Article in Biomaterials, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
- Osteomimetic microcalcification shapes the breast tumor microenvironment and metastatic potential.Cancer metastasis reviews · 2026Review
- A Correlative X‑ray Bioimaging Triad for Metals in Biomedical Research.Chemical & biomedical imaging · 2026Review
- Mineralized Cryogel/Hydrogel Constructs to Recapitulate Early Breast Cancer Bone Metastasis In Vitro.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Mechanisms of osteopontin-stabilized amorphous calcium phosphate calcification in benign and pre-malignant breast disease.Scientific reports · 2025Article
- Article
- Microcalcifications in breast cancer tissue studied by X-ray absorption, emission, scattering and diffraction.Journal of applied crystallography · 2025Article
- Article
- Exploration of utility of combined optical photothermal infrared and Raman imaging for investigating the chemical composition of microcalcifications in breast cancer.Analytical methods : advancing methods and applications · 2023Article
- Biomineralogical signatures of breast microcalcifications.Science advances · 2023Article
- Soft X-ray Fluorescence and Near-Edge Absorption Microscopy for Investigating Metabolic Features in Biological Systems: A Review.International journal of molecular sciences · 2023Review
- A time-course Raman spectroscopic analysis of spontaneous in vitro microcalcifications in a breast cancer cell line.Laboratory investigation; a journal of technical methods and pathology · 2021Article
- Multiple Pathways for Pathological Calcification in the Human Body.Advanced healthcare materials · 2021Review
- Obesity-associated Adipose Stromal Cells Promote Breast Cancer Invasion Through Direct Cell Contact and ECM Remodeling.Advanced functional materials · 2020Article
- Osteopontin in Bone Metabolism and Bone Diseases.Medical science monitor : international medical journal of experimental and clinical research · 2020Review
- Calcification Microstructure Reflects Breast Tissue Microenvironment.Journal of mammary gland biology and neoplasia · 2019Article
- Hydroxyapatite mineral enhances malignant potential in a tissue-engineered model of ductal carcinoma in situ (DCIS).Biomaterials · 2019Article
- Mapping and Profiling Lipid Distribution in a 3D Model of Breast Cancer Progression.ACS central science · 2019Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Microcalcifications serve as diagnostic markers for breast cancer, yet their formation pathway(s) and role in cancer progression are debated due in part to a lack of relevant 3D culture models that allow studying the extent of cellular regulation over mineralization. Previous studies have suggested processes ranging from dystrophic mineralization associated with cell death to bone-like mineral deposition. Here, we evaluated microcalcification formation in 3D multicellular spheroids, generated from non-malignant, pre-cancer, and invasive cell lines from the MCF10A human breast tumor progression series. The spheroids with greater malignancy potential developed necrotic cores, thus recapitulating spatially distinct viable and non-viable areas known to regulate cellular behavior in tumors in vivo. The spatial distribution of the microcalcifications, as well as their compositions, were characterized using nanoCT, electron-microscopy, and X-ray spectroscopy. Apatite microcalcifications were primarily detected within the viable cell regions and their number and size increased with malignancy potential of the spheroids. Levels of alkaline phosphatase decreased with malignancy potential, whereas levels of osteopontin increased. These findings support a mineralization pathway in which cancer cells induce mineralization in a manner that is linked to their malignancy potential, but that is distinct from physiological osteogenic mineralization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.